Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ACJX3V_RS01490 Genome accession   NZ_CP176523
Coordinates   268232..269353 (+) Length   373 a.a.
NCBI ID   WP_003156660.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MEPW12     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 263232..274353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJX3V_RS01470 - 263539..264534 (-) 996 WP_014304282.1 DUF5105 domain-containing protein -
  ACJX3V_RS01475 - 264663..266063 (+) 1401 WP_088037481.1 YcdB/YcdC domain-containing protein -
  ACJX3V_RS01480 - 266264..267622 (+) 1359 WP_003156662.1 YcdB/YcdC domain-containing protein -
  ACJX3V_RS01485 - 267619..268122 (-) 504 WP_072588311.1 M15 family metallopeptidase -
  ACJX3V_RS01490 rapF 268232..269353 (+) 1122 WP_003156660.1 tetratricopeptide repeat protein Regulator
  ACJX3V_RS01495 - 269469..270248 (+) 780 WP_015388737.1 glucose 1-dehydrogenase -
  ACJX3V_RS01500 - 270331..272010 (+) 1680 WP_043866811.1 glycoside hydrolase family 13 protein -
  ACJX3V_RS01505 - 272200..273159 (+) 960 WP_024084862.1 metal ABC transporter substrate-binding protein -
  ACJX3V_RS01510 - 273214..273909 (+) 696 WP_003156655.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 44333.58 Da        Isoelectric Point: 5.1691

>NTDB_id=967331 ACJX3V_RS01490 WP_003156660.1 268232..269353(+) (rapF) [Bacillus amyloliquefaciens strain MEPW12]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=967331 ACJX3V_RS01490 WP_003156660.1 268232..269353(+) (rapF) [Bacillus amyloliquefaciens strain MEPW12]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTAGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGAGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACAAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATCCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_12)

(187-251)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

42.588

99.464

0.424
  rapC Bacillus subtilis subsp. subtilis str. 168

40.323

99.732

0.402