Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKX34_RS24220 Genome accession   NZ_CP150232
Coordinates   5293224..5293655 (+) Length   143 a.a.
NCBI ID   WP_036683407.1    Uniprot ID   A0A1R0X267
Organism   Paenibacillus sp. FSL R5-0636     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5288224..5298655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX34_RS24185 (MKX34_24185) - 5288295..5288981 (-) 687 WP_076297508.1 M15 family metallopeptidase -
  MKX34_RS24190 (MKX34_24190) - 5289099..5289263 (+) 165 WP_172628852.1 hypothetical protein -
  MKX34_RS24195 (MKX34_24195) - 5289260..5289595 (-) 336 WP_076128863.1 YolD-like family protein -
  MKX34_RS24200 (MKX34_24200) - 5289811..5290371 (-) 561 WP_076297509.1 hypothetical protein -
  MKX34_RS24205 (MKX34_24205) - 5290489..5290977 (+) 489 WP_036683346.1 PH domain-containing protein -
  MKX34_RS24210 (MKX34_24210) - 5291051..5292526 (-) 1476 WP_038572819.1 glycosyl hydrolase -
  MKX34_RS24215 (MKX34_24215) - 5292701..5293117 (-) 417 WP_038572821.1 cytidine deaminase -
  MKX34_RS24220 (MKX34_24220) nucA/comI 5293224..5293655 (+) 432 WP_036683407.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKX34_RS24225 (MKX34_24225) - 5293737..5293886 (-) 150 WP_155288175.1 hypothetical protein -
  MKX34_RS24230 (MKX34_24230) - 5294036..5294212 (+) 177 WP_076098563.1 YjfB family protein -
  MKX34_RS24235 (MKX34_24235) - 5294376..5294927 (+) 552 WP_076142253.1 ImmA/IrrE family metallo-endopeptidase -
  MKX34_RS24240 (MKX34_24240) - 5294994..5295155 (+) 162 WP_155288176.1 hypothetical protein -
  MKX34_RS24245 (MKX34_24245) - 5295239..5295385 (-) 147 WP_170914301.1 hypothetical protein -
  MKX34_RS24250 (MKX34_24250) - 5295495..5295830 (+) 336 WP_076213969.1 hypothetical protein -
  MKX34_RS24255 (MKX34_24255) - 5296275..5296769 (+) 495 WP_076103793.1 sigma-70 family RNA polymerase sigma factor -
  MKX34_RS24260 (MKX34_24260) - 5296766..5297755 (+) 990 WP_081384049.1 hypothetical protein -
  MKX34_RS24265 (MKX34_24265) - 5297842..5298051 (+) 210 WP_076297510.1 hypothetical protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15898.68 Da        Isoelectric Point: 4.3968

>NTDB_id=967135 MKX34_RS24220 WP_036683407.1 5293224..5293655(+) (nucA/comI) [Paenibacillus sp. FSL R5-0636]
MKKWISSLIIVALLALASYWFEQNGEPTDPSSPSNSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCEEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=967135 MKX34_RS24220 WP_036683407.1 5293224..5293655(+) (nucA/comI) [Paenibacillus sp. FSL R5-0636]
GTGAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCAATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAGC
ATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGCAAAGAG
TCCTTAAAAGGGATCCCTACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTGAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTGGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R0X267

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.932

72.028

0.497

Multiple sequence alignment