Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST03_RS27930 Genome accession   NZ_CP150226
Coordinates   6495103..6495543 (-) Length   146 a.a.
NCBI ID   WP_042238459.1    Uniprot ID   A0A089K589
Organism   Paenibacillus sp. FSL R5-0912     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 6490103..6500543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST03_RS27880 (NST03_27880) - 6490342..6490593 (+) 252 WP_269322074.1 undecaprenyl-diphosphate phosphatase -
  NST03_RS27885 (NST03_27885) - 6490650..6490778 (+) 129 WP_269322073.1 hypothetical protein -
  NST03_RS27890 (NST03_27890) - 6491090..6491353 (-) 264 WP_231637706.1 hypothetical protein -
  NST03_RS27895 (NST03_27895) - 6491404..6491673 (+) 270 WP_042238465.1 hypothetical protein -
  NST03_RS27900 (NST03_27900) - 6491685..6492344 (-) 660 WP_197072973.1 hypothetical protein -
  NST03_RS27905 (NST03_27905) - 6492560..6492757 (+) 198 WP_042238463.1 hypothetical protein -
  NST03_RS27910 (NST03_27910) - 6493240..6493404 (-) 165 WP_156123296.1 hypothetical protein -
  NST03_RS27915 (NST03_27915) - 6493492..6494034 (-) 543 WP_042238460.1 ImmA/IrrE family metallo-endopeptidase -
  NST03_RS27920 (NST03_27920) - 6494178..6494354 (-) 177 WP_076243860.1 YjfB family protein -
  NST03_RS27925 (NST03_27925) - 6494728..6494865 (-) 138 WP_156118708.1 hypothetical protein -
  NST03_RS27930 (NST03_27930) nucA/comI 6495103..6495543 (-) 441 WP_042238459.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST03_RS27935 (NST03_27935) - 6495729..6497234 (+) 1506 WP_231637705.1 glycosyl hydrolase -
  NST03_RS27940 (NST03_27940) - 6497252..6497749 (-) 498 WP_042238456.1 PH domain-containing protein -
  NST03_RS27945 (NST03_27945) - 6497949..6498569 (+) 621 WP_042238454.1 hypothetical protein -
  NST03_RS27950 (NST03_27950) - 6498815..6499150 (+) 336 WP_042238452.1 YolD-like family protein -
  NST03_RS27955 (NST03_27955) - 6499187..6499348 (-) 162 WP_197072972.1 hypothetical protein -
  NST03_RS27960 (NST03_27960) - 6499601..6500287 (+) 687 WP_042238451.1 M15 family metallopeptidase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16274.15 Da        Isoelectric Point: 4.5248

>NTDB_id=966968 NST03_RS27930 WP_042238459.1 6495103..6495543(-) (nucA/comI) [Paenibacillus sp. FSL R5-0912]
MKAKKFITSLIIVVLLSLAAYYFEENGQLPSQTPSSDNSEVVRLEFPSGRYPETARHIQEAIRNGESSICTIDRNEAEEN
RKESLKGVPTKKGYDRDEWPMAMCAEGGAGADIEYITPSDNRGAGSWVGNQLEDFADGTRVEFMFK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=966968 NST03_RS27930 WP_042238459.1 6495103..6495543(-) (nucA/comI) [Paenibacillus sp. FSL R5-0912]
ATGAAAGCCAAAAAATTCATCACCAGTCTCATTATTGTTGTTCTGCTCAGCCTCGCTGCTTATTACTTCGAGGAGAACGG
TCAGCTCCCGTCCCAGACACCGTCTTCTGACAATTCGGAGGTTGTCCGGCTGGAATTCCCATCGGGCCGTTATCCGGAGA
CCGCCAGACATATTCAGGAAGCGATCAGGAACGGCGAGTCCTCCATCTGCACAATTGACCGGAATGAAGCCGAAGAGAAC
CGTAAGGAATCCCTGAAGGGCGTACCCACCAAAAAAGGTTATGACCGCGACGAATGGCCGATGGCGATGTGTGCAGAAGG
CGGAGCCGGCGCAGATATTGAATACATAACCCCCAGCGATAACCGCGGGGCTGGAAGCTGGGTCGGCAATCAGCTGGAGG
ACTTCGCAGACGGTACCCGCGTAGAGTTCATGTTTAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089K589

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.185

81.507

0.507

Multiple sequence alignment