Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST45_RS04485 Genome accession   NZ_CP150224
Coordinates   1044066..1044497 (-) Length   143 a.a.
NCBI ID   WP_036683407.1    Uniprot ID   A0A1R0X267
Organism   Paenibacillus sp. FSL R7-0163     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1039066..1049497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST45_RS04440 (NST45_04440) - 1039671..1039940 (-) 270 WP_339824996.1 hypothetical protein -
  NST45_RS04445 (NST45_04445) - 1039966..1040955 (-) 990 WP_081386857.1 hypothetical protein -
  NST45_RS04450 (NST45_04450) - 1040952..1041446 (-) 495 WP_076103793.1 sigma-70 family RNA polymerase sigma factor -
  NST45_RS04455 (NST45_04455) - 1041891..1042226 (-) 336 WP_076213969.1 hypothetical protein -
  NST45_RS04460 (NST45_04460) - 1042336..1042482 (+) 147 WP_170914301.1 hypothetical protein -
  NST45_RS04465 (NST45_04465) - 1042566..1042727 (-) 162 WP_155288176.1 hypothetical protein -
  NST45_RS04470 (NST45_04470) - 1042794..1043345 (-) 552 WP_076142253.1 ImmA/IrrE family metallo-endopeptidase -
  NST45_RS04475 (NST45_04475) - 1043509..1043685 (-) 177 WP_076098563.1 YjfB family protein -
  NST45_RS04480 (NST45_04480) - 1043835..1043984 (+) 150 WP_155288175.1 hypothetical protein -
  NST45_RS04485 (NST45_04485) nucA/comI 1044066..1044497 (-) 432 WP_036683407.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST45_RS04490 (NST45_04490) - 1044604..1045020 (+) 417 WP_076103796.1 cytidine deaminase -
  NST45_RS04495 (NST45_04495) - 1045194..1046669 (+) 1476 WP_339825002.1 glycosyl hydrolase -
  NST45_RS04500 (NST45_04500) - 1046743..1047231 (-) 489 WP_036683346.1 PH domain-containing protein -
  NST45_RS04505 (NST45_04505) - 1047349..1047909 (+) 561 WP_339825005.1 hypothetical protein -
  NST45_RS04510 (NST45_04510) - 1048124..1048459 (+) 336 WP_076142249.1 YolD-like family protein -
  NST45_RS04515 (NST45_04515) - 1048456..1048620 (-) 165 WP_169744799.1 hypothetical protein -
  NST45_RS04520 (NST45_04520) - 1048738..1049424 (+) 687 WP_036683338.1 M15 family metallopeptidase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15898.68 Da        Isoelectric Point: 4.3968

>NTDB_id=966876 NST45_RS04485 WP_036683407.1 1044066..1044497(-) (nucA/comI) [Paenibacillus sp. FSL R7-0163]
MKKWISSLIIVALLALASYWFEQNGEPTDPSSPSNSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCEEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=966876 NST45_RS04485 WP_036683407.1 1044066..1044497(-) (nucA/comI) [Paenibacillus sp. FSL R7-0163]
GTGAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCAATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAGC
ATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGCAAAGAG
TCCTTAAAAGGGATCCCTACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTGAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTGGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R0X267

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.932

72.028

0.497

Multiple sequence alignment