Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKY63_RS18020 Genome accession   NZ_CP150223
Coordinates   4152060..4152491 (-) Length   143 a.a.
NCBI ID   WP_036683407.1    Uniprot ID   A0A1R0X267
Organism   Paenibacillus sp. FSL R7-0189     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4147060..4157491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY63_RS17975 (MKY63_17975) - 4147675..4147932 (-) 258 WP_076275664.1 hypothetical protein -
  MKY63_RS17980 (MKY63_17980) - 4147958..4148947 (-) 990 WP_081389421.1 hypothetical protein -
  MKY63_RS17985 (MKY63_17985) - 4148944..4149438 (-) 495 WP_076275663.1 sigma-70 family RNA polymerase sigma factor -
  MKY63_RS17990 (MKY63_17990) - 4149884..4150219 (-) 336 WP_036683354.1 hypothetical protein -
  MKY63_RS17995 (MKY63_17995) - 4150329..4150475 (+) 147 WP_155288177.1 hypothetical protein -
  MKY63_RS18000 (MKY63_18000) - 4150559..4150720 (-) 162 WP_155288176.1 hypothetical protein -
  MKY63_RS18005 (MKY63_18005) - 4150787..4151338 (-) 552 WP_076275677.1 ImmA/IrrE family metallo-endopeptidase -
  MKY63_RS18010 (MKY63_18010) - 4151503..4151679 (-) 177 WP_076098563.1 YjfB family protein -
  MKY63_RS18015 (MKY63_18015) - 4151829..4151978 (+) 150 WP_155288175.1 hypothetical protein -
  MKY63_RS18020 (MKY63_18020) nucA/comI 4152060..4152491 (-) 432 WP_036683407.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKY63_RS18025 (MKY63_18025) - 4152598..4153014 (+) 417 WP_038572821.1 cytidine deaminase -
  MKY63_RS18030 (MKY63_18030) - 4153189..4154664 (+) 1476 WP_076275662.1 glycosyl hydrolase -
  MKY63_RS18035 (MKY63_18035) - 4154738..4155226 (-) 489 WP_036683346.1 PH domain-containing protein -
  MKY63_RS18040 (MKY63_18040) - 4155344..4155904 (+) 561 WP_038572817.1 hypothetical protein -
  MKY63_RS18045 (MKY63_18045) - 4156112..4156447 (+) 336 WP_036683341.1 YolD-like family protein -
  MKY63_RS18050 (MKY63_18050) - 4156444..4156608 (-) 165 WP_169744799.1 hypothetical protein -
  MKY63_RS18055 (MKY63_18055) - 4156726..4157412 (+) 687 WP_076275661.1 M15 family metallopeptidase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15898.68 Da        Isoelectric Point: 4.3968

>NTDB_id=966861 MKY63_RS18020 WP_036683407.1 4152060..4152491(-) (nucA/comI) [Paenibacillus sp. FSL R7-0189]
MKKWISSLIIVALLALASYWFEQNGEPTDPSSPSNSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCEEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=966861 MKY63_RS18020 WP_036683407.1 4152060..4152491(-) (nucA/comI) [Paenibacillus sp. FSL R7-0189]
GTGAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCAATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAGC
ATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGCAAAGAG
TCCTTAAAAGGGATCCCTACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTGAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTAGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R0X267

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.932

72.028

0.497

Multiple sequence alignment