Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKY63_RS13135 Genome accession   NZ_CP150223
Coordinates   3061159..3062526 (+) Length   455 a.a.
NCBI ID   WP_036680472.1    Uniprot ID   A0A1R0YRV5
Organism   Paenibacillus sp. FSL R7-0189     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3056159..3067526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY63_RS13115 (MKY63_13115) - 3056359..3056820 (+) 462 WP_036680465.1 CtsR family transcriptional regulator -
  MKY63_RS13120 (MKY63_13120) - 3056851..3057375 (+) 525 WP_036680467.1 UvrB/UvrC motif-containing protein -
  MKY63_RS13125 (MKY63_13125) - 3057404..3058468 (+) 1065 WP_036680469.1 protein arginine kinase -
  MKY63_RS13130 (MKY63_13130) clpC 3058504..3060972 (+) 2469 WP_036680471.1 ATP-dependent protease ATP-binding subunit ClpC -
  MKY63_RS13135 (MKY63_13135) radA 3061159..3062526 (+) 1368 WP_036680472.1 DNA repair protein RadA Machinery gene
  MKY63_RS13140 (MKY63_13140) disA 3062540..3063616 (+) 1077 WP_036680475.1 DNA integrity scanning diadenylate cyclase DisA -
  MKY63_RS13145 (MKY63_13145) pssA 3063681..3064418 (+) 738 WP_036680477.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  MKY63_RS13150 (MKY63_13150) - 3064596..3065684 (+) 1089 WP_036680481.1 PIN/TRAM domain-containing protein -
  MKY63_RS13155 (MKY63_13155) ispD 3065780..3066478 (+) 699 WP_076317118.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MKY63_RS13160 (MKY63_13160) ispF 3066475..3066963 (+) 489 WP_036680486.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49750.55 Da        Isoelectric Point: 6.4197

>NTDB_id=966854 MKY63_RS13135 WP_036680472.1 3061159..3062526(+) (radA) [Paenibacillus sp. FSL R7-0189]
MAKAKTKFFCTDCGYESPKWFGKCPGCQAWNSMVEETESVVKTQGMNAPIFHSKEKAQSIISIESDKEPRILTGIGELNR
VLGGGIVPGSLVLVGGDPGIGKSTLLLQTSHALTTQGLRVLYISGEESVRQTKLRADRLDALSTELYVLCETNMESIEEA
IEQIQPQFLVIDSIQTVFMPEVTSAPGSVTQVRECTTRFMRIAKIRGIATVLVGHVTKEGAIAGPRMLEHMVDCVLYFEG
ERHHTYRLLRAVKNRFGSTNEIGIFEMGEAGLTEVENPSELFLSERPLGVAGSTVVASMEGTRPVLVELQALVAATHFPS
PRRMCTGMDHQRMALIIAVLEKRMGMFLQNQDAYLNVAGGVKLDEPAIDLAVAISIASSFRDISTKPYDVFFGEVGLTGE
VRGVSRAEMRVKEAAKLGFRRVIMPEKSMKGWKHPQDIQIIGVSTVADALSVALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=966854 MKY63_RS13135 WP_036680472.1 3061159..3062526(+) (radA) [Paenibacillus sp. FSL R7-0189]
ATGGCTAAAGCAAAAACAAAATTTTTTTGCACCGACTGTGGATACGAATCGCCAAAATGGTTCGGGAAATGCCCAGGGTG
CCAGGCTTGGAACTCCATGGTGGAGGAAACGGAAAGCGTAGTCAAAACACAGGGAATGAATGCCCCTATTTTTCATAGTA
AAGAAAAGGCACAGTCGATCATAAGTATAGAAAGTGACAAGGAACCGCGCATACTGACAGGTATAGGTGAGCTTAACCGT
GTTCTCGGTGGGGGGATCGTACCAGGATCGCTGGTACTAGTAGGGGGAGACCCAGGAATCGGTAAATCTACGCTCTTATT
ACAGACCTCGCACGCCCTTACAACACAGGGATTGCGAGTACTGTATATCTCGGGCGAAGAATCGGTAAGGCAAACTAAGC
TGCGCGCGGATCGCCTCGATGCGTTGTCAACGGAGCTTTATGTACTATGTGAGACAAATATGGAAAGCATAGAAGAAGCG
ATCGAACAAATCCAGCCGCAATTTCTTGTCATCGATTCTATTCAGACCGTATTTATGCCAGAAGTAACGAGTGCGCCAGG
CAGCGTGACCCAGGTTCGGGAGTGTACGACAAGATTTATGCGTATCGCTAAGATTCGTGGAATTGCAACAGTTCTCGTTG
GTCATGTTACCAAGGAAGGGGCTATTGCTGGACCACGGATGCTTGAACATATGGTGGACTGCGTGCTGTATTTTGAGGGT
GAGCGGCATCATACGTATAGACTTTTGCGGGCTGTAAAAAACCGCTTTGGCTCTACGAATGAAATCGGCATTTTCGAAAT
GGGTGAAGCGGGGCTTACCGAAGTAGAGAATCCATCGGAGCTCTTTTTATCGGAACGTCCGCTTGGCGTGGCTGGTTCTA
CTGTGGTGGCAAGTATGGAGGGAACTAGACCTGTGCTTGTAGAACTTCAGGCACTTGTTGCTGCCACCCACTTCCCTTCC
CCACGGCGAATGTGCACAGGCATGGATCACCAGCGGATGGCACTTATTATCGCTGTACTCGAGAAACGGATGGGCATGTT
CCTGCAGAATCAGGATGCATACCTGAATGTTGCCGGGGGAGTAAAGCTGGATGAACCGGCGATTGATCTAGCTGTAGCTA
TTAGTATCGCCTCCAGTTTTCGTGATATTTCAACAAAACCTTACGACGTTTTCTTCGGAGAAGTAGGACTTACCGGTGAG
GTAAGAGGCGTTTCGCGCGCGGAAATGCGCGTTAAGGAGGCCGCAAAGCTTGGCTTCCGGCGGGTGATTATGCCTGAGAA
GAGCATGAAGGGATGGAAGCACCCGCAAGATATCCAGATTATTGGCGTCAGTACCGTAGCAGATGCACTATCGGTCGCGT
TAGATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R0YRV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.231

100

0.668
  radA Streptococcus mitis NCTC 12261

58.444

98.901

0.578
  radA Streptococcus pneumoniae Rx1

58.444

98.901

0.578
  radA Streptococcus pneumoniae D39

58.444

98.901

0.578
  radA Streptococcus pneumoniae R6

58.444

98.901

0.578
  radA Streptococcus pneumoniae TIGR4

58.444

98.901

0.578
  radA Streptococcus mitis SK321

58.444

98.901

0.578

Multiple sequence alignment