Detailed information NTG0966587

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACKN8U_RS05580
• Genome accession: NZ_CP176035
• Coordinates: 1187138..1189342 (-)
• Length: 734 a.a.
• NCBI ID: WP_140201821.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain L2181
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1182138..1194342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKN8U_RS05555 (ACKN8U_05555)	-	1182365..1182589 (+)	225	WP_024703419.1	hypothetical protein	-
ACKN8U_RS05560 (ACKN8U_05560)	hslU	1183005..1184336 (-)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-
ACKN8U_RS05565 (ACKN8U_05565)	hslV	1184360..1184911 (-)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
ACKN8U_RS05570 (ACKN8U_05570)	ftsN	1185076..1185621 (-)	546	WP_005481405.1	cell division protein FtsN	-
ACKN8U_RS05575 (ACKN8U_05575)	cytR	1185798..1186805 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
ACKN8U_RS05580 (ACKN8U_05580)	priA	1187138..1189342 (-)	2205	WP_140201821.1	primosomal protein N'	Machinery gene
ACKN8U_RS05585 (ACKN8U_05585)	rpmE	1189637..1189858 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
ACKN8U_RS05590 (ACKN8U_05590)	rpsJ	1190316..1190627 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
ACKN8U_RS05595 (ACKN8U_05595)	rplC	1190642..1191271 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
ACKN8U_RS05600 (ACKN8U_05600)	rplD	1191289..1191891 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
ACKN8U_RS05605 (ACKN8U_05605)	rplW	1191888..1192190 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
ACKN8U_RS05610 (ACKN8U_05610)	rplB	1192206..1193030 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
ACKN8U_RS05615 (ACKN8U_05615)	rpsS	1193052..1193330 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
ACKN8U_RS05620 (ACKN8U_05620)	rplV	1193341..1193673 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82255.83 Da
• Isoelectric Point: 8.5714

>NTDB_id=966587 ACKN8U_RS05580 WP_140201821.1 1187138..1189342(-) (priA) [Vibrio parahaemolyticus strain L2181]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLIWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAIKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEDKGWIERIEKKPAITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTSNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALESIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=966587 ACKN8U_RS05580 WP_140201821.1 1187138..1189342(-) (priA) [Vibrio parahaemolyticus strain L2181]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGATTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGATCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGATAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGCAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCCCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTTCTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGTTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGCACGCGCCGAAAAGGCAGCTTAGAAGACGCGTTGGAATCCATCCGTAAGGGTGAAT
ATCAAATTTTGATTGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAGGTCGCAGGACGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTTACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGTCAAGTTCGCTTTACCTTAGAATCGCACCCTTTATT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.243	100	0.436