Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACKN8R_RS15720 Genome accession   NZ_CP176031
Coordinates   3356277..3357743 (+) Length   488 a.a.
NCBI ID   WP_128413668.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain L2171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3351277..3362743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKN8R_RS15695 (ACKN8R_15700) - 3352397..3353362 (+) 966 WP_005455122.1 calcium/sodium antiporter -
  ACKN8R_RS15700 (ACKN8R_15705) kdsD 3353399..3354370 (+) 972 WP_005484881.1 arabinose-5-phosphate isomerase KdsD -
  ACKN8R_RS15705 (ACKN8R_15710) lptC 3354461..3355024 (+) 564 WP_005467805.1 LPS export ABC transporter periplasmic protein LptC -
  ACKN8R_RS15710 (ACKN8R_15715) lptA 3355005..3355499 (+) 495 WP_005467803.1 lipopolysaccharide transport periplasmic protein LptA -
  ACKN8R_RS15715 (ACKN8R_15720) lptB 3355501..3356226 (+) 726 WP_005467801.1 LPS export ABC transporter ATP-binding protein -
  ACKN8R_RS15720 (ACKN8R_15725) rpoN/rpoN1 3356277..3357743 (+) 1467 WP_128413668.1 RNA polymerase factor sigma-54 Machinery gene
  ACKN8R_RS15725 (ACKN8R_15730) hpf 3357768..3358055 (+) 288 WP_005467451.1 ribosome hibernation promoting factor -
  ACKN8R_RS15730 (ACKN8R_15735) ptsN 3358058..3358504 (+) 447 WP_005467453.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACKN8R_RS15735 (ACKN8R_15740) rapZ 3358519..3359382 (+) 864 WP_005468663.1 RNase adapter RapZ -
  ACKN8R_RS15740 (ACKN8R_15745) - 3359385..3359660 (+) 276 WP_005468661.1 HPr family phosphocarrier protein -
  ACKN8R_RS15745 (ACKN8R_15750) mgtE 3359880..3361235 (+) 1356 WP_005468659.1 magnesium transporter -
  ACKN8R_RS15750 (ACKN8R_15755) pmbA 3361312..3362655 (-) 1344 WP_005459755.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54866.72 Da        Isoelectric Point: 4.3441

>NTDB_id=966536 ACKN8R_RS15720 WP_128413668.1 3356277..3357743(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain L2171]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLEVEEGHEEPQANGEDKSALETADNSANEPTEIE
VPDSSDVIEKSEISSELEIDTTWDDVYSANTGSTGLALDDDMPVYQGETTESLHDYLMWQLDLTPFSETDRTIAIAIIDA
IDDYGYLTLSPEEIHESFDNEDIELDEVEAVRKRIQQFDPLGVASRNLQECLLLQLATFPEDTPWLVEAKMILADHIDHL
GNRDYKLVIKETKLKEADLREVLKLIQQLDPRPGSRITPDDTEYVIPDVSVFKDHGKWTVSINPDSIPKLKVNQQYAQLG
KGNSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDVALAVDMHESTISRVTTQKF
MHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAP
SSQRKRLL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=966536 ACKN8R_RS15720 WP_128413668.1 3356277..3357743(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain L2171]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCACAGTTGCAACAAGCGATTCGCTTGCTGCA
GTTGTCGACTCTCGATCTTCAACAAGAAATCCAAGAAGCGTTGGACTCAAACCCGCTATTGGAAGTTGAAGAAGGCCACG
AAGAACCTCAAGCAAATGGTGAAGACAAATCAGCACTCGAAACTGCTGACAACAGTGCCAACGAGCCAACAGAAATCGAA
GTTCCTGATAGTTCTGACGTCATCGAAAAATCCGAAATCAGCTCAGAGCTTGAAATCGACACAACTTGGGATGACGTCTA
CAGCGCAAATACGGGCAGTACCGGTTTAGCGTTAGACGATGATATGCCGGTCTACCAAGGCGAAACCACAGAATCGCTAC
ACGATTACCTGATGTGGCAACTCGACTTAACGCCTTTCAGTGAAACCGACCGTACGATTGCCATTGCCATCATTGATGCG
ATTGACGATTACGGCTATCTGACCCTATCTCCTGAAGAGATTCACGAAAGCTTCGACAACGAAGATATTGAGTTGGATGA
AGTCGAAGCCGTGCGCAAACGCATCCAACAGTTTGACCCGCTTGGTGTGGCATCGCGGAACCTGCAAGAATGCTTACTCC
TGCAACTTGCAACCTTCCCAGAAGACACGCCTTGGCTTGTAGAAGCCAAAATGATTCTTGCCGATCACATCGATCACCTT
GGTAATCGCGATTACAAACTGGTCATCAAAGAAACTAAGCTAAAAGAAGCGGATCTACGCGAAGTATTAAAACTGATCCA
ACAGTTAGATCCTCGTCCGGGCAGCCGCATTACCCCAGACGACACAGAATACGTCATTCCGGACGTATCGGTATTTAAAG
ATCATGGGAAATGGACCGTTTCCATCAACCCTGACAGCATTCCAAAGTTAAAAGTTAACCAACAATACGCTCAATTAGGC
AAAGGCAACAGTGCGGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCAAAATGGTTAATTAAGAGCTTAGAGAGCAG
AAACGAGACGCTACTCAAAGTTGCAAGATGTATTGTTGAACATCAACAAGATTTCTTCGAGTATGGTGAAGAAGCCATGA
AGCCAATGGTACTCAATGATGTAGCCTTGGCGGTGGACATGCATGAATCCACAATATCTCGTGTTACGACACAAAAGTTC
ATGCATACGCCACGTGGCATTTTTGAATTGAAGTACTTCTTCTCCAGCCACGTCAGTACAGACAATGGCGGAGAATGTTC
GTCAACAGCAATCCGCGCACTCATTAAGAAACTGGTCGCGGCAGAGAATACTGCTAAACCACTTAGTGACAGTAAGATTG
CTGCTCTTCTGGCTGACCAAGGGATTCAGGTCGCAAGACGTACGATAGCTAAATATCGCGAATCCTTGGGTATTGCCCCT
TCAAGTCAGCGTAAACGCCTACTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.444

100

0.459