Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACKQF3_RS01160 Genome accession   NZ_CP175806
Coordinates   252362..254566 (+) Length   734 a.a.
NCBI ID   WP_001960145.1    Uniprot ID   -
Organism   Vibrio cholerae strain N19-2759     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 247362..259566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKQF3_RS01140 (ACKQF3_01140) - 248442..249608 (-) 1167 WP_000213240.1 O-succinylhomoserine (thiol)-lyase -
  ACKQF3_RS01145 (ACKQF3_01145) metJ 249876..250193 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  ACKQF3_RS01150 (ACKQF3_01150) - 250304..251581 (-) 1278 WP_001278589.1 malic enzyme-like NAD(P)-binding protein -
  ACKQF3_RS01155 (ACKQF3_01155) rpmE 251889..252107 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  ACKQF3_RS01160 (ACKQF3_01160) priA 252362..254566 (+) 2205 WP_001960145.1 primosomal protein N' Machinery gene
  ACKQF3_RS01165 (ACKQF3_01165) cytR 254872..255879 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  ACKQF3_RS01170 (ACKQF3_01170) - 256051..256602 (+) 552 WP_032475230.1 SPOR domain-containing protein -
  ACKQF3_RS01175 (ACKQF3_01175) hslV 256739..257296 (+) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  ACKQF3_RS01180 (ACKQF3_01180) hslU 257362..258693 (+) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82096.42 Da        Isoelectric Point: 8.4848

>NTDB_id=966282 ACKQF3_RS01160 WP_001960145.1 252362..254566(+) (priA) [Vibrio cholerae strain N19-2759]
MRPTIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALTGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLSS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=966282 ACKQF3_RS01160 WP_001960145.1 252362..254566(+) (priA) [Vibrio cholerae strain N19-2759]
ATGCGACCCACCATTGCCCGAGTTTGCCTGCCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTACAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAACAAGGTTTCGGCTGTTTTTTATTAGAAGGGGTAACGGGCTCAGGTAAAACAGAGGTGTATCTTAACCTCATCACC
CCTGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACTCCACAAACCATCAATCGTTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTGATGCACTCGGCGCTCAACGACACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTATAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTCGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCACAGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGCCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTCACTTTAGTCGCGCTGTTGGATGTT
GATGGTTCACTGTATAGCAGCGATTTTCGCGCTTCTGAGCGCTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGTGAAGTGGTGTTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCTCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGTCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

DEAD/DEAH box helicase (DEAD)

(205-364)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.708

100

0.44