Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJJVF_RS18765 Genome accession   NZ_CP175682
Coordinates   3873117..3873557 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain Z1324PEC0010     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3868117..3878557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJVF_RS18745 aroP 3869115..3870488 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  ACJJVF_RS18750 ampE 3870531..3871385 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  ACJJVF_RS18755 ampD 3871382..3871933 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJJVF_RS18760 nadC 3872021..3872914 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJJVF_RS18765 pilA 3873117..3873557 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  ACJJVF_RS18770 pilB 3873567..3874952 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  ACJJVF_RS18775 hofC 3874942..3876144 (+) 1203 WP_000157236.1 protein transport protein HofC -
  ACJJVF_RS18780 guaC 3876179..3877222 (-) 1044 WP_001217338.1 GMP reductase -
  ACJJVF_RS18785 - 3877378..3877422 (-) 45 WP_120795372.1 protein YacM -
  ACJJVF_RS18790 coaE 3877447..3878067 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=966090 ACJJVF_RS18765 WP_000360895.1 3873117..3873557(+) (pilA) [Escherichia coli strain Z1324PEC0010]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=966090 ACJJVF_RS18765 WP_000360895.1 3873117..3873557(+) (pilA) [Escherichia coli strain Z1324PEC0010]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment