Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NSQ78_RS05270 Genome accession   NZ_CP150199
Coordinates   1132849..1134012 (+) Length   387 a.a.
NCBI ID   WP_042358565.1    Uniprot ID   A0A6N7NYV7
Organism   Stenotrophomonas sp. FSL W7-1447     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1127849..1139012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ78_RS05240 (NSQ78_05240) soxR 1127975..1128409 (+) 435 WP_053462342.1 redox-sensitive transcriptional activator SoxR -
  NSQ78_RS05245 (NSQ78_05245) - 1128478..1129080 (-) 603 WP_053462343.1 short chain dehydrogenase -
  NSQ78_RS05250 (NSQ78_05250) - 1129179..1130054 (+) 876 WP_064237775.1 LysR family transcriptional regulator -
  NSQ78_RS05255 (NSQ78_05255) proC 1130128..1130949 (-) 822 WP_064237776.1 pyrroline-5-carboxylate reductase -
  NSQ78_RS05260 (NSQ78_05260) - 1130968..1131645 (-) 678 WP_064237777.1 YggS family pyridoxal phosphate-dependent enzyme -
  NSQ78_RS05265 (NSQ78_05265) pilT 1131733..1132770 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  NSQ78_RS05270 (NSQ78_05270) pilU 1132849..1134012 (+) 1164 WP_042358565.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NSQ78_RS05275 (NSQ78_05275) - 1134048..1134776 (+) 729 WP_149901904.1 hypothetical protein -
  NSQ78_RS05280 (NSQ78_05280) - 1134888..1135871 (+) 984 WP_049469693.1 aldo/keto reductase -
  NSQ78_RS05285 (NSQ78_05285) - 1135914..1136813 (-) 900 WP_064237779.1 LysR family transcriptional regulator -
  NSQ78_RS05290 (NSQ78_05290) - 1136907..1137119 (+) 213 WP_064237780.1 DUF4287 domain-containing protein -
  NSQ78_RS05295 (NSQ78_05295) - 1137229..1137936 (+) 708 WP_049469684.1 YitT family protein -
  NSQ78_RS05300 (NSQ78_05300) - 1138021..1138566 (-) 546 WP_049469681.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43108.51 Da        Isoelectric Point: 6.5899

>NTDB_id=966052 NSQ78_RS05270 WP_042358565.1 1132849..1134012(+) (pilU) [Stenotrophomonas sp. FSL W7-1447]
MPSWEPAVNSTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=966052 NSQ78_RS05270 WP_042358565.1 1132849..1134012(+) (pilU) [Stenotrophomonas sp. FSL W7-1447]
ATGCCCTCTTGGGAGCCCGCCGTGAACAGCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CCCCACTGACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCTGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCCCCCGATGGTCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTCGGCACGCCGCTGGTGCAGGACTATATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGACTGGATGGCGTGGAGATCTCGGAGATCCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7NYV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395

Multiple sequence alignment