Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NSQ10_RS11865 Genome accession   NZ_CP150196
Coordinates   2314621..2315391 (-) Length   256 a.a.
NCBI ID   WP_039180043.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0432     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2309621..2320391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ10_RS11845 (NSQ10_11845) - 2309712..2310533 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  NSQ10_RS11850 (NSQ10_11850) - 2310559..2311197 (-) 639 WP_039180035.1 helix-turn-helix transcriptional regulator -
  NSQ10_RS11855 (NSQ10_11855) glyS 2311365..2313410 (-) 2046 WP_039180040.1 glycine--tRNA ligase subunit beta -
  NSQ10_RS11860 (NSQ10_11860) glyQ 2313403..2314290 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  NSQ10_RS11865 (NSQ10_11865) recO 2314621..2315391 (-) 771 WP_039180043.1 DNA repair protein RecO Machinery gene
  NSQ10_RS11870 (NSQ10_11870) - 2315427..2315570 (-) 144 WP_003217288.1 YqzL family protein -
  NSQ10_RS11875 (NSQ10_11875) era 2315719..2316624 (-) 906 WP_007501305.1 GTPase Era -
  NSQ10_RS11880 (NSQ10_11880) - 2316617..2317015 (-) 399 WP_008356263.1 cytidine deaminase -
  NSQ10_RS11885 (NSQ10_11885) - 2317129..2317515 (-) 387 WP_024423020.1 diacylglycerol kinase family protein -
  NSQ10_RS11890 (NSQ10_11890) ybeY 2317493..2317972 (-) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  NSQ10_RS11895 (NSQ10_11895) - 2317973..2320060 (-) 2088 WP_200885529.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.88 Da        Isoelectric Point: 8.1204

>NTDB_id=965923 NSQ10_RS11865 WP_039180043.1 2314621..2315391(-) (recO) [Bacillus sp. FSL R5-0432]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELIDKGTDEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=965923 NSQ10_RS11865 WP_039180043.1 2314621..2315391(-) (recO) [Bacillus sp. FSL R5-0432]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGTCAGCCGTTTT
TATACGGCACCTTTCTTATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTGATTGATAAAGGGACAGATGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTTCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGCTTTATTTGTCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAGCCTCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75

100

0.75

Multiple sequence alignment