Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST56_RS14250 Genome accession   NZ_CP150195
Coordinates   2680318..2680743 (-) Length   141 a.a.
NCBI ID   WP_010335025.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0560     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2675318..2685743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST56_RS14220 (NST56_14220) - 2675580..2675714 (-) 135 WP_268449338.1 hypothetical protein -
  NST56_RS14225 (NST56_14225) - 2675792..2676520 (-) 729 WP_010335020.1 SGNH/GDSL hydrolase family protein -
  NST56_RS14230 (NST56_14230) - 2676767..2677519 (-) 753 WP_339177292.1 N-acetylmuramoyl-L-alanine amidase -
  NST56_RS14235 (NST56_14235) - 2677714..2678340 (+) 627 WP_229146777.1 TVP38/TMEM64 family protein -
  NST56_RS14240 (NST56_14240) gnd 2678361..2679254 (-) 894 WP_339177295.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NST56_RS14245 (NST56_14245) - 2679556..2680284 (+) 729 WP_339177299.1 DUF308 domain-containing protein -
  NST56_RS14250 (NST56_14250) nucA/comI 2680318..2680743 (-) 426 WP_010335025.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST56_RS14255 (NST56_14255) sigK 2680919..2681647 (+) 729 WP_174754429.1 RNA polymerase sporulation sigma factor SigK -
  NST56_RS14260 (NST56_14260) - 2681776..2682053 (-) 278 Protein_2763 recombinase family protein -
  NST56_RS14265 (NST56_14265) - 2682590..2683528 (-) 939 WP_010335028.1 DMT family transporter -
  NST56_RS14270 (NST56_14270) - 2683798..2685273 (+) 1476 WP_339182239.1 PLP-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15484.63 Da        Isoelectric Point: 5.2215

>NTDB_id=965861 NST56_RS14250 WP_010335025.1 2680318..2680743(-) (nucA/comI) [Bacillus sp. FSL R5-0560]
MKRWMAMLLLAAAAFLCLLGKDMTAPQQVKGAALYDKVLYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=965861 NST56_RS14250 WP_010335025.1 2680318..2680743(-) (nucA/comI) [Bacillus sp. FSL R5-0560]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGCTTTTCTTTGTTTATTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAGTTTTATATTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGCTATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCGGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.29

87.943

0.539

Multiple sequence alignment