Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NST56_RS05900 Genome accession   NZ_CP150195
Coordinates   1179810..1183514 (+) Length   1234 a.a.
NCBI ID   WP_339180678.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0560     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1174810..1188514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST56_RS05885 (NST56_05885) - 1175285..1175599 (-) 315 WP_268446705.1 four-helix bundle copper-binding protein -
  NST56_RS05890 (NST56_05890) - 1175725..1176138 (-) 414 Protein_1094 rubrerythrin family protein -
  NST56_RS05895 (NST56_05895) addB 1176323..1179823 (+) 3501 WP_339180675.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NST56_RS05900 (NST56_05900) addA 1179810..1183514 (+) 3705 WP_339180678.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NST56_RS05905 (NST56_05905) sbcD 1183586..1184764 (+) 1179 WP_339180680.1 exonuclease subunit SbcD -
  NST56_RS05910 (NST56_05910) sbcC 1184761..1188153 (+) 3393 WP_339180682.1 exonuclease subunit SbcC -
  NST56_RS05915 (NST56_05915) hlpB 1188167..1188469 (+) 303 WP_229147779.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141559.07 Da        Isoelectric Point: 5.2219

>NTDB_id=965827 NST56_RS05900 WP_339180678.1 1179810..1183514(+) (addA) [Bacillus sp. FSL R5-0560]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEEHPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIRELPFYPYVKEDIAMVLEGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIEHQDDFNELYKRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGSGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYPKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAASDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVTPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTTEEAERTVRHLYEKELLTEEQQSAIDIEEIVQFFHTEIGE
KLIGAKWKDREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=965827 NST56_RS05900 WP_339180678.1 1179810..1183514(+) (addA) [Bacillus sp. FSL R5-0560]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGATCTGGTAAAACGGCTGTTCTTGTGGAAAGAATGATCCGGAAAATCACGGCGGAAGAACATC
CGATCGATGTAGACCGGCTTCTTGTTGTCACCTTTACAAACGCCTCAGCTGCAGAGATGAAACACCGGATTGCGGATGCA
CTGGAAAAAGAGCTGGTAAAACGCCCAGGTTCGCTGCATATCCGACGCCAGCTGTCTCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAACTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGCGATAAGGCG
TTTTTTGAGCTTGTCGACCGATACACAACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCGGATCCGGAAACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCA
GGGAACTGCCGTTTTATCCATATGTCAAAGAAGATATTGCGATGGTGCTGGAGGGAGCAAAGGAAAAGCTTCAGCGTGCT
CTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTGAGCATCAGGATGACTTCAATGAACTTTATAAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGACCCCGTCTTGCTTGATGAAGCGACAGACTTGAGAAATGGCGCCAAAAAACTCCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATTTAAAGAGTTTGGCTGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGGAAAAGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAGGGTGAACGTGAACCAAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTCGATGAATATCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAGCAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTCCTTT
TTCTGTCTAAGTACAAACGGTTTACAGAGAGCGGAGAAGGGTCGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAACTGAAACTCGGCGCGGCGTATCCGGAAAACAATGAAACGGAAACCGAGCTGCTGCTGATTGACAAGTCCG
AGGAGACGGACGCAGGAGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAAGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAAGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTCGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCATACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTTTATCCAAAGCTGCAAACGT
TTTACGGGCATTTGCAAAAATGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAGTATATGGATTATGTTGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCTAACTTGCGCGTCCTCTATGACCGGGC
ACGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGCTTAATGACCATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTTGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTATCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCTGAGGAATTGCGTGTGCTCTATGTTGCTTTAACGCGGGCAAAGGAGAAGCTGTTTCTT
GTCGGTTCATGCAAGGATCACCAGAAACAGCTGGCAAAATGGCAGGCATCGGCATCCCAAGCTGACTGGCTCCTGCCGGA
ATTTGATCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGTGCGGCAGCGGCTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGACGGGCCCCCGTTACACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAGAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGACAGAAGAAGCGGAGCGGACAGTGCGGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCGATATTGAGGAAATCGTTCAATTTTTCCATACCGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGGACCGTGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGACGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGGAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCATATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.436

100

0.934

Multiple sequence alignment