Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NST29_RS00520 Genome accession   NZ_CP150194
Coordinates   100181..101551 (+) Length   456 a.a.
NCBI ID   WP_033021064.1    Uniprot ID   -
Organism   Geobacillus sp. FSL W8-0026     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 95181..106551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST29_RS00500 (NST29_00500) - 95548..96009 (+) 462 WP_033021074.1 CtsR family transcriptional regulator -
  NST29_RS00505 (NST29_00505) - 96025..96573 (+) 549 WP_033021072.1 UvrB/UvrC motif-containing protein -
  NST29_RS00510 (NST29_00510) - 96578..97669 (+) 1092 WP_033021069.1 protein arginine kinase -
  NST29_RS00515 (NST29_00515) clpC 97666..100101 (+) 2436 WP_033021066.1 ATP-dependent protease ATP-binding subunit ClpC -
  NST29_RS00520 (NST29_00520) radA 100181..101551 (+) 1371 WP_033021064.1 DNA repair protein RadA Machinery gene
  NST29_RS00525 (NST29_00525) - 101738..102832 (+) 1095 WP_033842700.1 PIN/TRAM domain-containing protein -
  NST29_RS00530 (NST29_00530) ispD 102853..103539 (+) 687 WP_033842699.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NST29_RS00535 (NST29_00535) ispF 103555..104037 (+) 483 WP_172418727.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NST29_RS00540 (NST29_00540) gltX 104537..106009 (+) 1473 WP_033842698.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49502.97 Da        Isoelectric Point: 7.7489

>NTDB_id=965767 NST29_RS00520 WP_033021064.1 100181..101551(+) (radA) [Geobacillus sp. FSL W8-0026]
MVKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERAVSGRGAFFHTEPSSSAQPVPITAVTATQEPRIRTNSAELD
RVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAARHKVLYISGEESVKQVKLRAGRLHAESNELYVLAEANMEYIVT
AVETIQPACVIIDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSFG
NPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTHPADVIIGEVGLTG
EVRRVSRIEQRVQEAAKLGFSRVIVPKNNITGWQPPDGIQVIGVSHVAEALEHTMV

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=965767 NST29_RS00520 WP_033021064.1 100181..101551(+) (radA) [Geobacillus sp. FSL W8-0026]
ATGGTCAAAAAGAAAACGAAGTTTGTTTGTCAAGAGTGCGGTTATGAGTCGGCCAAATGGCTCGGCCGCTGTCCAGGATG
CCAAACGTGGAATTCGTTTGTGGAGGAAATCGAGCGGGCGGTTTCTGGGCGCGGCGCGTTTTTTCACACTGAACCGTCCA
GTTCGGCCCAACCGGTTCCGATTACGGCGGTCACAGCTACGCAAGAGCCGCGCATCCGAACGAACAGCGCGGAACTCGAT
CGCGTGCTCGGCGGCGGGATCGTCAAAGGTTCGCTCGTCTTGATCGGCGGTGACCCTGGGATTGGCAAATCGACGTTGCT
GCTGCAGACGTCGGCCCAGCTCGCCGCGGCCCGCCACAAGGTGTTGTATATATCCGGGGAGGAATCAGTGAAGCAGGTGA
AGCTGCGCGCCGGGCGCCTCCACGCCGAATCAAATGAACTATATGTGTTAGCCGAAGCGAATATGGAATATATTGTAACA
GCGGTGGAAACGATTCAGCCTGCCTGTGTGATCATCGATTCCATTCAGACGGTGTACCGGACCGATATTACGTCAGCCCC
GGGCAGCGTCGCCCAAGTGCGCGAATGTACGGCCGAGCTGATGAAAATCGCCAAAACGAAAGGCATCGCCATTTTCATTG
TCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCTTGCTTGAACATATGGTTGATACGGTTCTTTATTTTGAA
GGGGAGCGGCATCATACGTACCGTATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGCATTTTTGA
AATGCGCGACATCGGGCTGCGAGAGGTGGAAAACCCGTCGGAAGTGTTTTTGGAAGAGCGGTCGCGTGGAGCGGCGGGCT
CCACGGTCGTCGCGGCCATGGAAGGGACGCGCCCGGTGCTCGTTGAAATTCAGGCGCTCGTTTCGCCGACGAGTTTTGGC
AACCCAAGGCGAATGGCGACCGGCCTCGATCACAACCGCGTTTCGCTGCTGATGGCGGTGCTTGAAAAGCGGGTCGGGCT
GCTGTTGCAAAACCAAGACGCCTACTTGAAGGTAGCGGGCGGGGTAAAGCTTGACGAACCGGCCATTGACTTGGCGGTCG
CCGTCAGCATCGCCTCAAGTTTTCGCGACCGGCCGACCCATCCGGCCGATGTGATCATCGGCGAGGTTGGCTTGACCGGC
GAGGTGCGCCGTGTCTCGCGCATTGAACAACGGGTGCAAGAGGCGGCCAAACTAGGGTTTTCGCGCGTCATTGTGCCAAA
AAACAATATCACGGGCTGGCAGCCCCCCGACGGCATCCAAGTAATCGGCGTATCACATGTCGCTGAGGCCCTCGAGCATA
CGATGGTGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.465

100

0.735
  radA Streptococcus mitis NCTC 12261

65.342

99.342

0.649
  radA Streptococcus mitis SK321

65.265

99.123

0.647
  radA Streptococcus pneumoniae Rx1

65.044

99.123

0.645
  radA Streptococcus pneumoniae D39

65.044

99.123

0.645
  radA Streptococcus pneumoniae R6

65.044

99.123

0.645
  radA Streptococcus pneumoniae TIGR4

65.044

99.123

0.645

Multiple sequence alignment