Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSQ42_RS13035 Genome accession   NZ_CP150184
Coordinates   2514679..2515089 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. FSL W8-0812     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2509679..2520089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ42_RS13005 (NSQ42_13005) - 2509765..2509905 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NSQ42_RS13010 (NSQ42_13010) - 2510211..2510942 (-) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  NSQ42_RS13015 (NSQ42_13015) cwlH 2511195..2511947 (-) 753 WP_015714296.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NSQ42_RS13020 (NSQ42_13020) - 2512134..2512760 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  NSQ42_RS13025 (NSQ42_13025) gnd 2512779..2513672 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NSQ42_RS13030 (NSQ42_13030) - 2513924..2514646 (+) 723 WP_010886572.1 hypothetical protein -
  NSQ42_RS13035 (NSQ42_13035) nucA/comI 2514679..2515089 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NSQ42_RS13040 (NSQ42_13040) - 2515285..2515632 (+) 348 Protein_2519 sigma-70 family RNA polymerase sigma factor -
  NSQ42_RS13045 (NSQ42_13045) spoIVCA 2515663..2517123 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  NSQ42_RS13050 (NSQ42_13050) - 2517081..2517259 (-) 179 Protein_2521 hypothetical protein -
  NSQ42_RS13055 (NSQ42_13055) - 2517614..2518033 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  NSQ42_RS13060 (NSQ42_13060) acr3 2518045..2519085 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  NSQ42_RS13065 (NSQ42_13065) - 2519108..2519548 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  NSQ42_RS13070 (NSQ42_13070) arsR 2519609..2519926 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=965375 NSQ42_RS13035 WP_009967785.1 2514679..2515089(-) (nucA/comI) [Bacillus sp. FSL W8-0812]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=965375 NSQ42_RS13035 WP_009967785.1 2514679..2515089(-) (nucA/comI) [Bacillus sp. FSL W8-0812]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment