Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSU16_RS12765 Genome accession   NZ_CP150176
Coordinates   2497907..2498332 (-) Length   141 a.a.
NCBI ID   WP_339191046.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1003     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2492907..2503332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSU16_RS12740 (NSU16_12740) - 2493377..2494108 (-) 732 WP_229146778.1 SGNH/GDSL hydrolase family protein -
  NSU16_RS12745 (NSU16_12745) - 2494355..2495107 (-) 753 WP_168748283.1 N-acetylmuramoyl-L-alanine amidase -
  NSU16_RS12750 (NSU16_12750) - 2495302..2495928 (+) 627 WP_229146777.1 TVP38/TMEM64 family protein -
  NSU16_RS12755 (NSU16_12755) gnd 2495949..2496842 (-) 894 WP_339191041.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NSU16_RS12760 (NSU16_12760) - 2497145..2497873 (+) 729 WP_339191044.1 DUF308 domain-containing protein -
  NSU16_RS12765 (NSU16_12765) nucA/comI 2497907..2498332 (-) 426 WP_339191046.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NSU16_RS12770 (NSU16_12770) sigK 2498509..2499237 (+) 729 WP_174754429.1 RNA polymerase sporulation sigma factor SigK -
  NSU16_RS12775 (NSU16_12775) - 2499366..2499534 (-) 169 Protein_2466 recombinase family protein -
  NSU16_RS12780 (NSU16_12780) - 2499492..2499670 (-) 179 Protein_2467 hypothetical protein -
  NSU16_RS12785 (NSU16_12785) - 2499811..2500966 (-) 1156 Protein_2468 IS3 family transposase -
  NSU16_RS12790 (NSU16_12790) - 2501100..2502305 (-) 1206 WP_339191049.1 MFS transporter -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15468.63 Da        Isoelectric Point: 5.2215

>NTDB_id=965016 NSU16_RS12765 WP_339191046.1 2497907..2498332(-) (nucA/comI) [Bacillus sp. FSL K6-1003]
MKRWMAMLLLAAAAFLCLLGKDMTAPQQVKGAALYDKVLYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGFDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=965016 NSU16_RS12765 WP_339191046.1 2497907..2498332(-) (nucA/comI) [Bacillus sp. FSL K6-1003]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGCTTTTCTTTGTTTATTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAGTTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATTTGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGTTTTGATCGAGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGCTATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCGGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532

Multiple sequence alignment