Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NSU16_RS05745 Genome accession   NZ_CP150176
Coordinates   1129299..1133003 (+) Length   1234 a.a.
NCBI ID   WP_339192278.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1003     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1124299..1138003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSU16_RS05730 (NSU16_05730) - 1124774..1125088 (-) 315 WP_010333659.1 four-helix bundle copper-binding protein -
  NSU16_RS05735 (NSU16_05735) - 1125214..1125627 (-) 414 Protein_1063 rubrerythrin family protein -
  NSU16_RS05740 (NSU16_05740) addB 1125812..1129312 (+) 3501 WP_339192277.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSU16_RS05745 (NSU16_05745) addA 1129299..1133003 (+) 3705 WP_339192278.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSU16_RS05750 (NSU16_05750) sbcD 1133075..1134253 (+) 1179 WP_339180680.1 exonuclease subunit SbcD -
  NSU16_RS05755 (NSU16_05755) sbcC 1134250..1137642 (+) 3393 WP_339192279.1 exonuclease subunit SbcC -
  NSU16_RS05760 (NSU16_05760) hlpB 1137656..1137958 (+) 303 WP_229147779.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141616.09 Da        Isoelectric Point: 5.1862

>NTDB_id=964982 NSU16_RS05745 WP_339192278.1 1129299..1133003(+) (addA) [Bacillus sp. FSL K6-1003]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEEHPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
FELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAASDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTVGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAASDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAYEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVTPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTTEEAERTVQHLYEKELLTEEQQSAIDIEEIVQFFHTEIGG
KLICAKWKDREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=964982 NSU16_RS05745 WP_339192278.1 1129299..1133003(+) (addA) [Bacillus sp. FSL K6-1003]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGATCTGGTAAAACGGCTGTTCTTGTGGAAAGAATGATCCGAAAAATCACGGCGGAAGAACATC
CGATTGATGTAGACCGGCTTCTTGTTGTCACCTTTACAAACGCCTCAGCTGCAGAGATGAAACACCGGATTGCGGATGCA
CTGGAAAAAGAGCTGGTAAAACGCCCAGGTTCGCTGCATATCCGACGCCAGCTGTCTCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAACTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGCGATAAGGCG
TTTTTTGAGCTTGTCGACCGATACACAACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCAGATCCGGAAACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCATATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTTCAGCGTGCT
TTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCACGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATAAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGATGAGTTTGACCCCGTCTTGCTTGATGAAGCGACAGACTTGAGAAATGGCGCCAAAAAACTCCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATTTGAAAAGTTTGGCTGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGTGAACGTGAACCAAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTCGATGAATATCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAGCAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTCCTTT
TTCTGTCCAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAACTGAAACTCGGCGCGGCGTATCCGGAAAACAATGAAACGGAAACCGAACTGCTGCTGATTGACAAGTCCG
AGGAGACGGACGCAGGAGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTCGAGGCAAAAGCGATCGCCAAGGAAATACGT
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATTGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAAGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCTCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCAGCGACCGAAACGATGAGCTTTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAATGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAGTATATGGATTATGTTGGCGGAATGCCGGGCGGCAAACAGCGGCAGGCTAACTTGCGCGTCCTCTATGACCGGGC
ACGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAAGATGTTGTCCGCTTAATGACCATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGTGGGACTTGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTATCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCTGAGGAATTGCGTGTGCTCTATGTTGCTTTAACGCGGGCAAAGGAGAAGCTGTTTCTT
GTCGGCTCATGCAAGGATCACCAGAAACAGCTGGCAAAATGGCAGGCATCGGCATCCCAAGCTGACTGGCTCCTGCCGGA
ATTTGATCGCTATCAGGCGAAAACGTATTTGGATTTCATTGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGTGCGGCAGCGGCTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAGCGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATATGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTGTCTGAGATTAAGAGAAGAAGAGAGTACGAGGACGAATACAGCGGACGGGCCCCCGTTACACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAGAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGACAGAAGAAGCAGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCGATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGCGGA
AAACTGATCTGCGCTAAATGGAAGGACCGTGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATTATCGATTGTCTCTACGAAACAGAGGACGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGGAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCATATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.436

100

0.934

Multiple sequence alignment