Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSS75_RS13725 Genome accession   NZ_CP150175
Coordinates   2652619..2653044 (-) Length   141 a.a.
NCBI ID   WP_101861068.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1012     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2647619..2658044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS75_RS13695 (NSS75_13695) - 2647858..2647992 (-) 135 WP_010335019.1 hypothetical protein -
  NSS75_RS13700 (NSS75_13700) - 2648070..2648801 (-) 732 WP_024122120.1 SGNH/GDSL hydrolase family protein -
  NSS75_RS13705 (NSS75_13705) - 2649048..2649800 (-) 753 WP_185848806.1 N-acetylmuramoyl-L-alanine amidase -
  NSS75_RS13710 (NSS75_13710) - 2649995..2650621 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  NSS75_RS13715 (NSS75_13715) gnd 2650643..2651536 (-) 894 WP_101861066.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NSS75_RS13720 (NSS75_13720) - 2651857..2652585 (+) 729 WP_213417751.1 DUF308 domain-containing protein -
  NSS75_RS13725 (NSS75_13725) nucA/comI 2652619..2653044 (-) 426 WP_101861068.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NSS75_RS13730 (NSS75_13730) - 2653221..2653571 (+) 351 Protein_2657 sigma-70 family RNA polymerase sigma factor -
  NSS75_RS13735 (NSS75_13735) spoIVCA 2653599..2655059 (-) 1461 WP_339237641.1 site-specific DNA recombinase SpoIVCA -
  NSS75_RS13740 (NSS75_13740) - 2655017..2655195 (-) 179 Protein_2659 hypothetical protein -
  NSS75_RS13745 (NSS75_13745) - 2655852..2657369 (+) 1518 WP_339233391.1 class I adenylate-forming enzyme family protein -
  NSS75_RS13750 (NSS75_13750) - 2657414..2657743 (-) 330 Protein_2661 excinuclease ABC subunit UvrA -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15470.65 Da        Isoelectric Point: 5.7516

>NTDB_id=964935 NSS75_RS13725 WP_101861068.1 2652619..2653044(-) (nucA/comI) [Bacillus sp. FSL K6-1012]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQAKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRGGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=964935 NSS75_RS13725 WP_101861068.1 2652619..2653044(-) (nucA/comI) [Bacillus sp. FSL K6-1012]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGCCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGGTGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCAACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTGA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532

Multiple sequence alignment