Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NSS75_RS06040 Genome accession   NZ_CP150175
Coordinates   1177534..1181238 (+) Length   1234 a.a.
NCBI ID   WP_339236513.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1012     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1172534..1186238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS75_RS06025 (NSS75_06025) - 1172983..1173297 (-) 315 WP_339236505.1 four-helix bundle copper-binding protein -
  NSS75_RS06030 (NSS75_06030) - 1173426..1173863 (-) 438 WP_326231138.1 ferritin family protein -
  NSS75_RS06035 (NSS75_06035) addB 1174047..1177547 (+) 3501 WP_339236510.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSS75_RS06040 (NSS75_06040) addA 1177534..1181238 (+) 3705 WP_339236513.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSS75_RS06045 (NSS75_06045) sbcD 1181310..1182488 (+) 1179 WP_024120840.1 exonuclease subunit SbcD -
  NSS75_RS06050 (NSS75_06050) sbcC 1182485..1185877 (+) 3393 WP_339236516.1 exonuclease subunit SbcC -
  NSS75_RS06055 (NSS75_06055) - 1185891..1186193 (+) 303 WP_101860849.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141512.09 Da        Isoelectric Point: 5.1869

>NTDB_id=964901 NSS75_RS06040 WP_339236513.1 1177534..1181238(+) (addA) [Bacillus sp. FSL K6-1012]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDELIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTNGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETGLLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRVFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALTEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSYHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGAKWKNREIPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIKLYTRAVGQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=964901 NSS75_RS06040 WP_339236513.1 1177534..1181238(+) (addA) [Bacillus sp. FSL K6-1012]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGATTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCGGGATCACTGCATATCCGACGCCAGCTGTCGCTGTTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGCTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTACGAGTA
TTCCCGTTCCCATCCTGATCCGGAGACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTGCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGAGCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAACGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAGG
GGGATGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTAAGAAATGGCGCCAAAAAACTGCTTGAAAAACTTAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTCCA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCGAAGCAGGAGAAATCGCTTATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTTGTCGATGAATATCAGGATACCAACCTGGTGCAAGAATCCATTCTTCAGCTCGTCACAAACGGGCC
GGAAGAGACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTTTCAAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTCTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAACGAAACGGAAACAGGGCTGCTGCTGATTGACAAGTCAG
AGGAGGCGGACGCAGGCGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCTAAAGCGATCGCTAAAGAAATACGC
AAGCTGATCTCATCGCCATTTAAAGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCGATGCCGTGGGCGCCGCAAATCATGGAGGAACTGCGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTAATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTCTACCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGTTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGATTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ATCTGGGAACGGCGAGAGCGTTGACCGAACAGGAGGATGTTGTCCGTTTGATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCAGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAATTGCTGTCTGAGGAATTGCGTGTCCTCTATGTTGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCGAAATGGCAGGCATCCGCATCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGTGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGTTTTGCTATTCAAATGATTCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGGGAGCGGCTGGCTTGGAAGTATTCGTATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGACGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATCGAGGAAATCGTTCAATTTTTCCATACAGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGAACCGCGAAATTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGAATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTAAGCTGTACACAAGGGCCGTTGGACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.112

100

0.931

Multiple sequence alignment