Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NSS75_RS00580 Genome accession   NZ_CP150175
Coordinates   105590..106966 (+) Length   458 a.a.
NCBI ID   WP_044153447.1    Uniprot ID   A0A9Q2LD59
Organism   Bacillus sp. FSL K6-1012     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100590..111966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS75_RS00560 (NSS75_00560) ctsR 100943..101407 (+) 465 WP_339235309.1 transcriptional regulator CtsR -
  NSS75_RS00565 (NSS75_00565) mcsA 101421..101978 (+) 558 WP_103672506.1 protein-arginine kinase activator protein McsA -
  NSS75_RS00570 (NSS75_00570) - 101978..103069 (+) 1092 WP_059291687.1 protein arginine kinase -
  NSS75_RS00575 (NSS75_00575) clpC 103066..105498 (+) 2433 WP_010332750.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  NSS75_RS00580 (NSS75_00580) radA 105590..106966 (+) 1377 WP_044153447.1 DNA repair protein RadA Machinery gene
  NSS75_RS00585 (NSS75_00585) disA 106970..108052 (+) 1083 WP_003242042.1 DNA integrity scanning diadenylate cyclase DisA -
  NSS75_RS00590 (NSS75_00590) - 108172..109272 (+) 1101 WP_059291688.1 PIN/TRAM domain-containing protein -
  NSS75_RS00595 (NSS75_00595) ispD 109287..109985 (+) 699 WP_268498699.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NSS75_RS00600 (NSS75_00600) ispF 109978..110454 (+) 477 WP_095714845.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49532.07 Da        Isoelectric Point: 8.2443

>NTDB_id=964885 NSS75_RS00580 WP_044153447.1 105590..106966(+) (radA) [Bacillus sp. FSL K6-1012]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSTRHVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIEEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=964885 NSS75_RS00580 WP_044153447.1 105590..106966(+) (radA) [Bacillus sp. FSL K6-1012]
ATGGCTAAAACAAAATCCAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACCGTACAGAAACCTTCCCCCATAACATCAATCGAAACATCAGAAGAACCCCGGGTTAAAACACAGCTCGGCGAGTTT
AACAGAGTGCTCGGCGGCGGTGTGGTGAAGGGCTCACTTGTTTTAATTGGCGGGGACCCCGGCATCGGAAAATCGACCTT
ATTACTGCAAGTATCCGCTCAGTTATCTGGTTCAACACGTCATGTTCTGTACATTTCAGGAGAAGAATCGGTAAAACAAA
CTAAATTAAGAGCAGACCGTCTGGGCATAAATAATCCCTCACTGCATGTTTTATCCGAAACCGATATGGAGTATATTTCG
TCTGCTATAGAAGAGATGAATCCAGCGTTTGTGGTTGTTGACTCTATTCAAACGGTCTATCAAAGCGATATTACATCGGC
TCCCGGCAGTGTGTCACAGGTAAGAGAATGTACAGCCGAGCTGATGAAAATTGCAAAGACAAAAGGTATTCCGATATTTA
TTGTAGGTCACGTAACGAAAGAAGGATCCATCGCGGGGCCGAGACTGCTGGAGCATATGGTTGACACTGTTTTATACTTT
GAAGGAGAACGTCACCATACTTTCCGAATCTTACGGGCTGTAAAAAACCGCTTTGGTTCTACAAACGAAATGGGTATTTT
TGAAATGCGGGAAGAAGGGCTTACTGAGGTACTGAATCCTTCGGAAATCTTCTTAGAAGAGCGTTCGGCGGGATCTGCGG
GGTCGAGCATAACGGCTTCTATGGAAGGTACAAGGCCGATTCTTGTAGAAATTCAGGCACTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGCATGGCAACAGGGATAGATCATAACAGGGTGTCACTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCATATTTGAAGGTAGCGGGCGGCGTCAAACTTGATGAACCTGCGATTGATCTTGCTA
TAGCGGTCAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATAGGAGAAGTGGGATTAACA
GGAGAAGTCCGCCGGGTATCAAGAATTGAACAGCGTGTAAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
TGCGGCAAATCTGGATGGATGGACGAAACCAAAAGGGATTGAGGTCATCGGAGTAGCAAATGTTGCAGAGGCACTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.472

100

0.985
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment