Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NSS75_RS00020 Genome accession   NZ_CP150175
Coordinates   3033..4145 (+) Length   370 a.a.
NCBI ID   WP_024123582.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1012     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1534..28053 3033..4145 within 0


Gene organization within MGE regions


Location: 1534..28053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS75_RS00010 (NSS75_00010) dnaN 1534..2670 (+) 1137 WP_024123581.1 DNA polymerase III subunit beta -
  NSS75_RS00015 (NSS75_00015) rlbA 2802..3017 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  NSS75_RS00020 (NSS75_00020) recF 3033..4145 (+) 1113 WP_024123582.1 DNA replication/repair protein RecF Machinery gene
  NSS75_RS00025 (NSS75_00025) remB 4163..4408 (+) 246 WP_024123583.1 extracellular matrix regulator RemB -
  NSS75_RS00030 (NSS75_00030) gyrB 4464..6380 (+) 1917 WP_024123584.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NSS75_RS00035 (NSS75_00035) gyrA 6592..9063 (+) 2472 WP_024123585.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -
  NSS75_RS00065 (NSS75_00065) - 14381..15331 (-) 951 WP_116429902.1 YaaC family protein -
  NSS75_RS00070 (NSS75_00070) guaB 15452..16918 (+) 1467 WP_024123587.1 IMP dehydrogenase -
  NSS75_RS00075 (NSS75_00075) dacA 17073..18404 (+) 1332 WP_024123588.1 D-alanyl-D-alanine carboxypeptidase -
  NSS75_RS00080 (NSS75_00080) pdxS 18601..19485 (+) 885 WP_024123589.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  NSS75_RS00085 (NSS75_00085) pdxT 19506..20096 (+) 591 WP_024123590.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  NSS75_RS00090 (NSS75_00090) serS 20428..21705 (+) 1278 WP_059293409.1 serine--tRNA ligase -
  NSS75_RS00100 (NSS75_00100) dck 22045..22698 (-) 654 WP_024123592.1 deoxyadenosine/deoxycytidine kinase -
  NSS75_RS00105 (NSS75_00105) dgk 22695..23318 (-) 624 WP_326366405.1 deoxyguanosine kinase -
  NSS75_RS00110 (NSS75_00110) - 23417..24700 (-) 1284 WP_038954334.1 glycoside hydrolase family 18 protein -
  NSS75_RS00115 (NSS75_00115) - 24763..25314 (-) 552 WP_024123595.1 isochorismatase family cysteine hydrolase -
  NSS75_RS00120 (NSS75_00120) tadA 25398..25883 (+) 486 WP_024123596.1 tRNA adenosine(34) deaminase TadA -
  NSS75_RS00130 (NSS75_00130) dnaX 26359..28053 (+) 1695 WP_024123597.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42434.44 Da        Isoelectric Point: 7.0882

>NTDB_id=964881 NSS75_RS00020 WP_024123582.1 3033..4145(+) (recF) [Bacillus sp. FSL K6-1012]
MYIQNLELTSYRNYEHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLYDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALEVSDPEDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=964881 NSS75_RS00020 WP_024123582.1 3033..4145(+) (recF) [Bacillus sp. FSL K6-1012]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTATGAACATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATTGGAGAAAATGCTCAGGGGAAAACAAACCTCATGGAAGCGATCTATGTCTTGTCCATGGCAAAATCGCATCGGA
CTTCAAATGACAAAGAACTTATCCGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCAATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATCGAACAGCAAAAGCTCAGCCAGTA
TGTCGGCGCCCTCAACACCATCATGTTTGCACCGGAAGATTTAAACCTTGTAAAGGGAAGCCCTCAAGTGAGACGGAGAT
TTCTTGACATGGAAATCGGCCAAGTTTCTCCTGTCTACCTGTATGACCTTTCTCTTTACCAGAAGATTCTTTCCCAGCGA
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACAGAACAATGCTTGATGTTCTGACTGATCAGCTTATTGA
AGTTGCCGCAAAAGTCGTCGTAAAACGTCTGCAGTTCACGGCACAGCTCGAAAAATGGGCGCAGCCTATCCATTCGGGCA
TCTCAAGAGGGCTTGAGGAATTGACGCTGAAGTACCATACGGCGCTTGAGGTATCAGATCCTGAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATCGAGCGCGGCGTCACGCTGTCTGGACCTCATCG
GGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGGACGACGGCATTAT
CCCTTAAGCTGGCTGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTGCTGAGT
GAGCTGGATGATTATCGCCAATCGCACTTGCTTCATACCATCCAGGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGGCAAGCAGGAATGTTCCGTGTGCAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

98.108

100

0.981

Multiple sequence alignment