Detailed information NTG0964562

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACJEH3_RS11165
• Genome accession: NZ_CP174245
• Coordinates: 2471647..2473824 (-)
• Length: 725 a.a.
• NCBI ID: WP_010948552.1
• Uniprot ID: Q5ZRL5
• Organism: Legionella pneumophila strain SPF544
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2466647..2478824

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEH3_RS11140 (ACJEH3_11140)	-	2468411..2469217 (+)	807	WP_010948556.1	sulfite exporter TauE/SafE family protein	-
ACJEH3_RS11145 (ACJEH3_11145)	lgt	2469235..2470005 (+)	771	WP_010948555.1	prolipoprotein diacylglyceryl transferase	-
ACJEH3_RS11150 (ACJEH3_11150)	thyA	2470002..2470796 (+)	795	WP_010948554.1	thymidylate synthase	-
ACJEH3_RS11155 (ACJEH3_11155)	-	2470872..2471141 (+)	270	WP_016357044.1	hypothetical protein	-
ACJEH3_RS11160 (ACJEH3_11160)	-	2471258..2471650 (-)	393	WP_010948553.1	thioesterase family protein	-
ACJEH3_RS11165 (ACJEH3_11165)	priA	2471647..2473824 (-)	2178	WP_010948552.1	primosomal protein N'	Machinery gene
ACJEH3_RS11170 (ACJEH3_11170)	-	2473825..2474319 (-)	495	WP_010948551.1	6-carboxytetrahydropterin synthase	-
ACJEH3_RS11175 (ACJEH3_11175)	-	2474322..2475434 (-)	1113	WP_015443911.1	class I SAM-dependent methyltransferase	-
ACJEH3_RS11180 (ACJEH3_11180)	-	2475611..2476366 (+)	756	WP_015443912.1	pteridine reductase	-
ACJEH3_RS11185 (ACJEH3_11185)	lgt2	2476492..2478402 (+)	1911	WP_010948548.1	Dot/Icm type IV secretion system effector glucosyltransferase Lgt2/LegC8	-

Sequence

Protein

• Length: 725 a.a.
• Molecular weight: 81788.11 Da
• Isoelectric Point: 9.1746

>NTDB_id=964562 ACJEH3_RS11165 WP_010948552.1 2471647..2473824(-) (priA) [Legionella pneumophila strain SPF544]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide

• Length: 2178 bp

>NTDB_id=964562 ACJEH3_RS11165 WP_010948552.1 2471647..2473824(-) (priA) [Legionella pneumophila strain SPF544]
ATGAACGATATTTATCAGGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGACCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTCGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCAAAAAAATATCGCCTGGGTTTACC
CTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATACCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAGTTGATTGATTTTTTAAGCAGGCACCAGAAAGGGATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCAATTAACCTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGCTAACACTCAATCCTGAACAAGCCGTTGCCGTAGATTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGGAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCACCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTACCCAATCTTGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTACAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCGTTACACTATCAACTGATTGATTTACGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGTGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGTAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGTCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGAGCAATTCCTAAAAGCAACCAAAGATCAAATTCAAGCTCATCCTCTCACGGTACTGGGTCC
TGCACCAGCTCCTTTACCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (442-468)

• Helicase conserved C-terminal domain (Helicase_C) (489-585)

• Primosomal protein N C-terminal domain (PriA_C) (629-721)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-99)

• Type III restriction enzyme, res subunit (ResIII) (195-357)

3D structure

• AlphaFold DB: Q5ZRL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	42.219	100	0.43