Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NYE66_RS00515 Genome accession   NZ_CP150167
Coordinates   99064..100434 (+) Length   456 a.a.
NCBI ID   WP_339239909.1    Uniprot ID   -
Organism   Geobacillus sp. FSL W8-0466     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94064..105434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE66_RS00495 (NYE66_00495) ctsR 94436..94897 (+) 462 WP_033017268.1 transcriptional regulator CtsR -
  NYE66_RS00500 (NYE66_00500) - 94912..95460 (+) 549 WP_033008611.1 UvrB/UvrC motif-containing protein -
  NYE66_RS00505 (NYE66_00505) - 95465..96556 (+) 1092 WP_033017267.1 protein arginine kinase -
  NYE66_RS00510 (NYE66_00510) clpC 96553..98988 (+) 2436 WP_033017266.1 ATP-dependent protease ATP-binding subunit ClpC -
  NYE66_RS00515 (NYE66_00515) radA 99064..100434 (+) 1371 WP_339239909.1 DNA repair protein RadA Machinery gene
  NYE66_RS00520 (NYE66_00520) - 100720..101814 (+) 1095 WP_049624211.1 PIN/TRAM domain-containing protein -
  NYE66_RS00525 (NYE66_00525) ispD 101836..102522 (+) 687 WP_033008621.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NYE66_RS00530 (NYE66_00530) ispF 102537..103019 (+) 483 WP_033008623.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NYE66_RS00535 (NYE66_00535) gltX 103126..104595 (+) 1470 WP_049624214.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49349.86 Da        Isoelectric Point: 7.7488

>NTDB_id=964537 NYE66_RS00515 WP_339239909.1 99064..100434(+) (radA) [Geobacillus sp. FSL W8-0466]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIEPVRTAVRGAFLHSEPSGSAKPVPITAVAAVQEPRIETNSAEF
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQASAQLAARHTVLYVSGEESVKQVKLRAGRLRAESDHLYVLAEANLEYIVS
AIETIQPACVVIDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGVAIFIVGHVTKEGAIAGPRILEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSHGAAGSTVVAAMEGTRPVLVEIQALVSPTSFG
NPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDRPTNPTDVIIGEVGLTG
EVRRVSRIEQRVQEAVKLGFSRVIVPKNNLAGWQPPASVQVIGVSHVAEALEHTMI

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=964537 NYE66_RS00515 WP_339239909.1 99064..100434(+) (radA) [Geobacillus sp. FSL W8-0466]
ATGGCGAAAAAGAAGACGAAATTTGTTTGCCAAGAGTGCGGATACGAATCGGCGAAGTGGCTCGGCCGCTGCCCGGGATG
CCAAACGTGGAACTCATTCGTCGAGGAAATCGAGCCGGTCAGAACCGCTGTGCGTGGGGCATTTCTCCATTCGGAGCCGT
CCGGGTCCGCTAAACCGGTTCCGATTACTGCCGTTGCCGCGGTGCAAGAGCCGCGCATCGAAACGAACAGTGCAGAGTTC
AATCGCGTGCTCGGTGGGGGGATCGTCAAAGGGTCGCTCGTCTTAATCGGCGGCGACCCCGGCATCGGCAAGTCGACGCT
GTTGCTGCAGGCCTCAGCGCAACTGGCGGCTAGGCATACAGTGTTGTATGTATCGGGCGAGGAATCCGTCAAACAGGTAA
AACTGCGCGCTGGGCGCCTTCGCGCCGAGTCCGATCATCTGTATGTGTTAGCGGAGGCGAATTTGGAATATATTGTATCA
GCGATTGAAACGATTCAGCCCGCCTGTGTCGTGATTGATTCAATCCAGACGGTGTACCGGACGGATATTACGTCAGCGCC
GGGCAGTGTCGCCCAAGTGCGCGAGTGCACCGCTGAGTTGATGAAAATCGCCAAAACAAAAGGCGTCGCCATTTTTATCG
TCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCATTCTCGAGCATATGGTCGATACCGTCCTTTATTTTGAG
GGGGAGCGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGTATTTTTGA
AATGCGCGACATCGGGCTGCGGGAAGTGGAAAATCCGTCCGAAGTGTTTTTGGAAGAGCGATCGCACGGGGCGGCGGGTT
CGACGGTGGTGGCGGCCATGGAAGGGACGCGCCCGGTTCTGGTGGAAATTCAAGCGCTCGTTTCGCCGACAAGCTTCGGC
AACCCGAGGCGGATGGCGACCGGCCTCGATCATAACCGCGTGTCGTTGCTTATGGCCGTATTGGAAAAGCGGGTCGGGCT
GCTGTTGCAAAACCAAGATGCCTATTTGAAGGTGGCGGGCGGAGTGAAGCTTGATGAGCCGGCCATTGACCTAGCCATCG
CCGTCAGCATCGCCTCAAGCTTTCGCGATCGGCCGACCAATCCGACCGACGTCATTATCGGTGAAGTCGGGTTGACCGGG
GAAGTGCGGCGTGTCTCCCGCATTGAACAGCGTGTGCAAGAGGCTGTCAAACTAGGTTTTTCCCGTGTCATTGTACCGAA
AAACAATTTGGCAGGTTGGCAGCCGCCGGCAAGCGTTCAGGTGATCGGCGTTTCCCATGTGGCCGAGGCGCTCGAGCACA
CGATGATTTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-221)

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.961

100

0.741
  radA Streptococcus mitis NCTC 12261

64.68

99.342

0.643
  radA Streptococcus mitis SK321

64.68

99.342

0.643
  radA Streptococcus pneumoniae Rx1

64.459

99.342

0.64
  radA Streptococcus pneumoniae D39

64.459

99.342

0.64
  radA Streptococcus pneumoniae R6

64.459

99.342

0.64
  radA Streptococcus pneumoniae TIGR4

64.459

99.342

0.64

Multiple sequence alignment