Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NST88_RS12650 Genome accession   NZ_CP150162
Coordinates   2464863..2465246 (-) Length   127 a.a.
NCBI ID   WP_003230168.1    Uniprot ID   -
Organism   Bacillus sp. PS68     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459863..2470246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST88_RS12610 (NST88_12610) sinI 2460797..2460970 (+) 174 WP_003230187.1 anti-repressor SinI family protein Regulator
  NST88_RS12615 (NST88_12615) sinR 2461004..2461339 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  NST88_RS12620 (NST88_12620) tasA 2461432..2462217 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  NST88_RS12625 (NST88_12625) - 2462281..2462853 (-) 573 WP_003230181.1 signal peptidase I -
  NST88_RS12630 (NST88_12630) tapA 2462837..2463598 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  NST88_RS12635 (NST88_12635) - 2463870..2464196 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NST88_RS12640 (NST88_12640) - 2464238..2464417 (-) 180 WP_003230176.1 YqzE family protein -
  NST88_RS12645 (NST88_12645) comGG 2464488..2464862 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  NST88_RS12650 (NST88_12650) comGF 2464863..2465246 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  NST88_RS12655 (NST88_12655) comGE 2465272..2465619 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  NST88_RS12660 (NST88_12660) comGD 2465603..2466034 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  NST88_RS12665 (NST88_12665) comGC 2466024..2466320 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NST88_RS12670 (NST88_12670) comGB 2466334..2467371 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  NST88_RS12675 (NST88_12675) comGA 2467358..2468428 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NST88_RS12680 (NST88_12680) corA 2468840..2469793 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14281.37 Da        Isoelectric Point: 5.8929

>NTDB_id=964236 NST88_RS12650 WP_003230168.1 2464863..2465246(-) (comGF) [Bacillus sp. PS68]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=964236 NST88_RS12650 WP_003230168.1 2464863..2465246(-) (comGF) [Bacillus sp. PS68]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
GGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAAGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment