Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJEHH_RS06810 Genome accession   NZ_CP174238
Coordinates   1531258..1532652 (+) Length   464 a.a.
NCBI ID   WP_027220222.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF587     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1526258..1537652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHH_RS06785 (ACJEHH_06785) ankC 1526986..1528473 (-) 1488 WP_011214726.1 Dot/Icm T4SS effector AnkC/LegA12 -
  ACJEHH_RS06790 (ACJEHH_06790) - 1528551..1529675 (-) 1125 WP_011214725.1 M42 family metallopeptidase -
  ACJEHH_RS06795 (ACJEHH_06795) trmB 1529687..1530364 (-) 678 WP_011214724.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJEHH_RS06800 (ACJEHH_06800) rpmB 1530633..1530869 (+) 237 WP_010946227.1 50S ribosomal protein L28 -
  ACJEHH_RS06805 (ACJEHH_06805) rpmG 1530884..1531048 (+) 165 WP_003635328.1 50S ribosomal protein L33 -
  ACJEHH_RS06810 (ACJEHH_06810) rpoN/rpoN1 1531258..1532652 (+) 1395 WP_027220222.1 RNA polymerase factor sigma-54 Machinery gene
  ACJEHH_RS06815 (ACJEHH_06815) hpf 1532679..1532978 (+) 300 WP_010946225.1 ribosome hibernation promoting factor -
  ACJEHH_RS06820 (ACJEHH_06820) - 1533169..1533438 (+) 270 WP_010946224.1 HPr family phosphocarrier protein -
  ACJEHH_RS06825 (ACJEHH_06825) pssA 1533685..1534422 (-) 738 WP_408073107.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACJEHH_RS06830 (ACJEHH_06830) - 1534652..1534936 (+) 285 WP_011945638.1 DUF3579 domain-containing protein -
  ACJEHH_RS06835 (ACJEHH_06835) dotV 1535080..1535622 (-) 543 WP_010946221.1 type IV secretion system protein DotV -
  ACJEHH_RS06840 (ACJEHH_06840) - 1535706..1536440 (+) 735 WP_408073105.1 ferredoxin--NADP reductase -
  ACJEHH_RS06845 (ACJEHH_06845) - 1536590..1537600 (+) 1011 WP_408073103.1 class I fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52967.28 Da        Isoelectric Point: 5.4456

>NTDB_id=964075 ACJEHH_RS06810 WP_027220222.1 1531258..1532652(+) (rpoN/rpoN1) [Legionella pneumophila strain SPF587]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPIAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=964075 ACJEHH_RS06810 WP_027220222.1 1531258..1532652(+) (rpoN/rpoN1) [Legionella pneumophila strain SPF587]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAGCAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACCAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCTCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTTGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCATTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTGGGATGCGCATCTGTTAATTTAGCAGAAACTCTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCGAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTAACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGTCAATATGCCGCTTTAATCC
AACGTGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTACAAGAAGCACGTTGGTTTTTAAAAAGTATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTAGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAGCGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

39.087

100

0.425

Multiple sequence alignment