Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST79_RS13400 Genome accession   NZ_CP150159
Coordinates   2574970..2575380 (-) Length   136 a.a.
NCBI ID   WP_212060327.1    Uniprot ID   -
Organism   Bacillus sp. PS196     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2569970..2580380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST79_RS13370 (NST79_13370) - 2570057..2570197 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NST79_RS13375 (NST79_13375) - 2570503..2571234 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NST79_RS13380 (NST79_13380) cwlH 2571486..2572238 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NST79_RS13385 (NST79_13385) - 2572425..2573051 (+) 627 WP_033884461.1 TVP38/TMEM64 family protein -
  NST79_RS13390 (NST79_13390) gnd 2573070..2573963 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NST79_RS13395 (NST79_13395) - 2574215..2574937 (+) 723 WP_010886572.1 hypothetical protein -
  NST79_RS13400 (NST79_13400) nucA/comI 2574970..2575380 (-) 411 WP_212060327.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST79_RS13405 (NST79_13405) - 2575576..2575926 (+) 351 Protein_2592 sigma-70 family RNA polymerase sigma factor -
  NST79_RS13410 (NST79_13410) - 2576066..2576536 (+) 471 WP_033884465.1 MarR family transcriptional regulator -
  NST79_RS13415 (NST79_13415) - 2576687..2578099 (+) 1413 WP_116316519.1 MDR family MFS transporter -
  NST79_RS13420 (NST79_13420) spoIVCA 2578286..2579746 (-) 1461 WP_277737219.1 site-specific DNA recombinase SpoIVCA -
  NST79_RS13425 (NST79_13425) - 2579704..2579882 (-) 179 Protein_2596 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14982.00 Da        Isoelectric Point: 5.1853

>NTDB_id=964002 NST79_RS13400 WP_212060327.1 2574970..2575380(-) (nucA/comI) [Bacillus sp. PS196]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEAHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=964002 NST79_RS13400 WP_212060327.1 2574970..2575380(-) (nucA/comI) [Bacillus sp. PS196]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGCACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(42-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.739

84.559

0.522