Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJEH2_RS10135 Genome accession   NZ_CP174235
Coordinates   2266641..2268035 (-) Length   464 a.a.
NCBI ID   WP_027220222.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF590     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2261641..2273035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH2_RS10100 (ACJEH2_10100) - 2261693..2262703 (-) 1011 WP_408073103.1 class I fructose-bisphosphate aldolase -
  ACJEH2_RS10105 (ACJEH2_10105) - 2262853..2263587 (-) 735 WP_408073105.1 ferredoxin--NADP reductase -
  ACJEH2_RS10110 (ACJEH2_10110) dotV 2263671..2264213 (+) 543 WP_010946221.1 type IV secretion system protein DotV -
  ACJEH2_RS10115 (ACJEH2_10115) - 2264357..2264641 (-) 285 WP_011945638.1 DUF3579 domain-containing protein -
  ACJEH2_RS10120 (ACJEH2_10120) pssA 2264871..2265608 (+) 738 WP_408073107.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACJEH2_RS10125 (ACJEH2_10125) - 2265855..2266124 (-) 270 WP_010946224.1 HPr family phosphocarrier protein -
  ACJEH2_RS10130 (ACJEH2_10130) hpf 2266315..2266614 (-) 300 WP_010946225.1 ribosome hibernation promoting factor -
  ACJEH2_RS10135 (ACJEH2_10135) rpoN/rpoN1 2266641..2268035 (-) 1395 WP_027220222.1 RNA polymerase factor sigma-54 Machinery gene
  ACJEH2_RS10140 (ACJEH2_10140) rpmG 2268245..2268409 (-) 165 WP_003635328.1 50S ribosomal protein L33 -
  ACJEH2_RS10145 (ACJEH2_10145) rpmB 2268424..2268660 (-) 237 WP_010946227.1 50S ribosomal protein L28 -
  ACJEH2_RS10150 (ACJEH2_10150) trmB 2268929..2269606 (+) 678 WP_011214724.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJEH2_RS10155 (ACJEH2_10155) - 2269618..2270742 (+) 1125 WP_011214725.1 M42 family metallopeptidase -
  ACJEH2_RS10160 (ACJEH2_10160) ankC 2270820..2272307 (+) 1488 WP_011214726.1 Dot/Icm T4SS effector AnkC/LegA12 -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52967.28 Da        Isoelectric Point: 5.4456

>NTDB_id=963882 ACJEH2_RS10135 WP_027220222.1 2266641..2268035(-) (rpoN/rpoN1) [Legionella pneumophila strain SPF590]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPIAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=963882 ACJEH2_RS10135 WP_027220222.1 2266641..2268035(-) (rpoN/rpoN1) [Legionella pneumophila strain SPF590]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAGCAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACCAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCTCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTTGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCATTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTGGGATGCGCATCTGTTAATTTAGCAGAAACTCTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCGAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTAACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGTCAATATGCCGCTTTAATCC
AACGTGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTACAAGAAGCACGTTGGTTTTTAAAAAGTATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTAGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAGCGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

39.087

100

0.425