Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSQ83_RS13045 Genome accession   NZ_CP150157
Coordinates   2531131..2531541 (-) Length   136 a.a.
NCBI ID   WP_198878615.1    Uniprot ID   -
Organism   Bacillus sp. PS217     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2526131..2536541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ83_RS13015 (NSQ83_13015) - 2526218..2526358 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NSQ83_RS13020 (NSQ83_13020) - 2526664..2527395 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  NSQ83_RS13025 (NSQ83_13025) cwlH 2527647..2528399 (-) 753 WP_128993171.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NSQ83_RS13030 (NSQ83_13030) - 2528586..2529212 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  NSQ83_RS13035 (NSQ83_13035) gnd 2529231..2530124 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NSQ83_RS13040 (NSQ83_13040) - 2530376..2531098 (+) 723 WP_010886572.1 hypothetical protein -
  NSQ83_RS13045 (NSQ83_13045) nucA/comI 2531131..2531541 (-) 411 WP_198878615.1 sporulation-specific Dnase NucB Machinery gene
  NSQ83_RS13050 (NSQ83_13050) - 2531737..2532084 (+) 348 Protein_2521 sigma-70 family RNA polymerase sigma factor -
  NSQ83_RS13055 (NSQ83_13055) spoIVCA 2532115..2533575 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  NSQ83_RS13060 (NSQ83_13060) - 2533533..2533711 (-) 179 Protein_2523 hypothetical protein -
  NSQ83_RS13065 (NSQ83_13065) - 2534066..2534485 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  NSQ83_RS13070 (NSQ83_13070) acr3 2534497..2535537 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  NSQ83_RS13075 (NSQ83_13075) - 2535560..2536000 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  NSQ83_RS13080 (NSQ83_13080) arsR 2536061..2536378 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14968.92 Da        Isoelectric Point: 5.1853

>NTDB_id=963840 NSQ83_RS13045 WP_198878615.1 2531131..2531541(-) (nucA/comI) [Bacillus sp. PS217]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFNVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=963840 NSQ83_RS13045 WP_198878615.1 2531131..2531541(-) (nucA/comI) [Bacillus sp. PS217]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
ATGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529