Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NSQ16_RS12915 Genome accession   NZ_CP150155
Coordinates   2504146..2504556 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. PS108     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2499146..2509556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ16_RS12885 (NSQ16_12885) - 2499233..2499373 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NSQ16_RS12890 (NSQ16_12890) - 2499679..2500410 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  NSQ16_RS12895 (NSQ16_12895) cwlH 2500662..2501414 (-) 753 WP_160221685.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NSQ16_RS12900 (NSQ16_12900) - 2501601..2502227 (+) 627 WP_160221686.1 TVP38/TMEM64 family protein -
  NSQ16_RS12905 (NSQ16_12905) gnd 2502246..2503139 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NSQ16_RS12910 (NSQ16_12910) - 2503391..2504113 (+) 723 WP_010886572.1 hypothetical protein -
  NSQ16_RS12915 (NSQ16_12915) nucA/comI 2504146..2504556 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NSQ16_RS12920 (NSQ16_12920) - 2504752..2505099 (+) 348 Protein_2495 sigma-70 family RNA polymerase sigma factor -
  NSQ16_RS12925 (NSQ16_12925) spoIVCA 2505130..2506590 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  NSQ16_RS12930 (NSQ16_12930) - 2506548..2506726 (-) 179 Protein_2497 hypothetical protein -
  NSQ16_RS12935 (NSQ16_12935) - 2507081..2507500 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  NSQ16_RS12940 (NSQ16_12940) acr3 2507512..2508552 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  NSQ16_RS12945 (NSQ16_12945) - 2508575..2509015 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  NSQ16_RS12950 (NSQ16_12950) arsR 2509076..2509393 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=963676 NSQ16_RS12915 WP_009967785.1 2504146..2504556(-) (nucA/comI) [Bacillus sp. PS108]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=963676 NSQ16_RS12915 WP_009967785.1 2504146..2504556(-) (nucA/comI) [Bacillus sp. PS108]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529