Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST68_RS22695 Genome accession   NZ_CP150153
Coordinates   5116886..5117317 (+) Length   143 a.a.
NCBI ID   WP_036683407.1    Uniprot ID   A0A1R0X267
Organism   Paenibacillus sp. FSL E2-0230     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5111886..5122317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST68_RS22660 (NST68_22660) - 5111959..5112645 (-) 687 WP_036683338.1 M15 family metallopeptidase -
  NST68_RS22665 (NST68_22665) - 5112763..5112927 (+) 165 WP_169744799.1 hypothetical protein -
  NST68_RS22670 (NST68_22670) - 5112924..5113259 (-) 336 WP_076142249.1 YolD-like family protein -
  NST68_RS22675 (NST68_22675) - 5113474..5114034 (-) 561 WP_076098564.1 hypothetical protein -
  NST68_RS22680 (NST68_22680) - 5114152..5114640 (+) 489 WP_036683346.1 PH domain-containing protein -
  NST68_RS22685 (NST68_22685) - 5114714..5116189 (-) 1476 WP_038572819.1 glycosyl hydrolase -
  NST68_RS22690 (NST68_22690) - 5116363..5116779 (-) 417 WP_038572821.1 cytidine deaminase -
  NST68_RS22695 (NST68_22695) nucA/comI 5116886..5117317 (+) 432 WP_036683407.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST68_RS22700 (NST68_22700) - 5117399..5117548 (-) 150 WP_155288175.1 hypothetical protein -
  NST68_RS22705 (NST68_22705) - 5117698..5117874 (+) 177 WP_076098563.1 YjfB family protein -
  NST68_RS22710 (NST68_22710) - 5118038..5118589 (+) 552 WP_076142253.1 ImmA/IrrE family metallo-endopeptidase -
  NST68_RS22715 (NST68_22715) - 5118656..5118817 (+) 162 WP_155288176.1 hypothetical protein -
  NST68_RS22720 (NST68_22720) - 5118901..5119047 (-) 147 WP_170914301.1 hypothetical protein -
  NST68_RS22725 (NST68_22725) - 5119157..5119492 (+) 336 WP_036683354.1 hypothetical protein -
  NST68_RS22730 (NST68_22730) - 5119938..5120432 (+) 495 WP_036683356.1 sigma-70 family RNA polymerase sigma factor -
  NST68_RS22735 (NST68_22735) - 5120429..5121418 (+) 990 WP_036683358.1 hypothetical protein -
  NST68_RS22740 (NST68_22740) - 5121505..5121696 (+) 192 WP_076142250.1 hypothetical protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15898.68 Da        Isoelectric Point: 4.3968

>NTDB_id=963574 NST68_RS22695 WP_036683407.1 5116886..5117317(+) (nucA/comI) [Paenibacillus sp. FSL E2-0230]
MKKWISSLIIVALLALASYWFEQNGEPTDPSSPSNSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCEEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=963574 NST68_RS22695 WP_036683407.1 5116886..5117317(+) (nucA/comI) [Paenibacillus sp. FSL E2-0230]
GTGAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCAATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAGC
ATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGCAAAGAG
TCCTTAAAAGGGATCCCTACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTGAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTGGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R0X267

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.932

72.028

0.497