Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   NSS98_RS22820 Genome accession   NZ_CP150152
Coordinates   5147051..5147482 (+) Length   143 a.a.
NCBI ID   WP_036683407.1    Uniprot ID   A0A1R0X267
Organism   Paenibacillus sp. FSL E2-0274     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5133957..5153214 5147051..5147482 within 0


Gene organization within MGE regions


Location: 5133957..5153214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS98_RS22740 (NSS98_22740) - 5133957..5134844 (-) 888 WP_076315988.1 sugar ABC transporter permease -
  NSS98_RS22745 (NSS98_22745) - 5135683..5136738 (-) 1056 WP_076315990.1 LacI family DNA-binding transcriptional regulator -
  NSS98_RS22750 (NSS98_22750) - 5136863..5138107 (-) 1245 WP_076315991.1 hypothetical protein -
  NSS98_RS22755 (NSS98_22755) - 5138484..5138873 (+) 390 WP_076315992.1 sigma factor-like helix-turn-helix DNA-binding protein -
  NSS98_RS22760 (NSS98_22760) - 5138943..5139152 (+) 210 WP_076315993.1 helix-turn-helix domain-containing protein -
  NSS98_RS22765 (NSS98_22765) - 5139642..5139911 (+) 270 WP_076315994.1 helix-turn-helix domain-containing protein -
  NSS98_RS22770 (NSS98_22770) - 5139989..5141194 (+) 1206 WP_076315995.1 site-specific integrase -
  NSS98_RS22780 (NSS98_22780) - 5141608..5142048 (-) 441 WP_036683335.1 WGxxGxxG family protein -
  NSS98_RS22785 (NSS98_22785) - 5142124..5142810 (-) 687 WP_036683338.1 M15 family metallopeptidase -
  NSS98_RS22790 (NSS98_22790) - 5142928..5143092 (+) 165 WP_169744799.1 hypothetical protein -
  NSS98_RS22795 (NSS98_22795) - 5143089..5143424 (-) 336 WP_076142249.1 YolD-like family protein -
  NSS98_RS22800 (NSS98_22800) - 5143639..5144199 (-) 561 WP_076098564.1 hypothetical protein -
  NSS98_RS22805 (NSS98_22805) - 5144317..5144805 (+) 489 WP_036683346.1 PH domain-containing protein -
  NSS98_RS22810 (NSS98_22810) - 5144879..5146354 (-) 1476 WP_038572819.1 glycosyl hydrolase -
  NSS98_RS22815 (NSS98_22815) - 5146528..5146944 (-) 417 WP_038572821.1 cytidine deaminase -
  NSS98_RS22820 (NSS98_22820) nucA/comI 5147051..5147482 (+) 432 WP_036683407.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NSS98_RS22825 (NSS98_22825) - 5147564..5147713 (-) 150 WP_155288175.1 hypothetical protein -
  NSS98_RS22830 (NSS98_22830) - 5147863..5148039 (+) 177 WP_076098563.1 YjfB family protein -
  NSS98_RS22835 (NSS98_22835) - 5148203..5148754 (+) 552 WP_076142253.1 ImmA/IrrE family metallo-endopeptidase -
  NSS98_RS22840 (NSS98_22840) - 5148821..5148982 (+) 162 WP_155288176.1 hypothetical protein -
  NSS98_RS22845 (NSS98_22845) - 5149066..5149212 (-) 147 WP_170914301.1 hypothetical protein -
  NSS98_RS22850 (NSS98_22850) - 5149322..5149657 (+) 336 WP_036683354.1 hypothetical protein -
  NSS98_RS22855 (NSS98_22855) - 5150103..5150597 (+) 495 WP_036683356.1 sigma-70 family RNA polymerase sigma factor -
  NSS98_RS22860 (NSS98_22860) - 5150594..5151583 (+) 990 WP_036683358.1 hypothetical protein -
  NSS98_RS22865 (NSS98_22865) - 5151670..5151861 (+) 192 WP_076142250.1 hypothetical protein -
  NSS98_RS22870 (NSS98_22870) - 5152540..5153214 (+) 675 WP_036683363.1 hypothetical protein -

Sequence


Protein


Download         Length: 143 a.a.        Molecular weight: 15898.68 Da        Isoelectric Point: 4.3968

>NTDB_id=963539 NSS98_RS22820 WP_036683407.1 5147051..5147482(+) (nucA/comI) [Paenibacillus sp. FSL E2-0274]
MKKWISSLIIVALLALASYWFEQNGEPTDPSSPSNSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCEEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=963539 NSS98_RS22820 WP_036683407.1 5147051..5147482(+) (nucA/comI) [Paenibacillus sp. FSL E2-0274]
GTGAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCAATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAGC
ATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGCAAAGAG
TCCTTAAAAGGGATCCCTACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTGAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTGGAATTTATGTTTAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1R0X267

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.932

72.028

0.497