Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | NST94_RS22295 | Genome accession | NZ_CP150146 |
| Coordinates | 5136307..5136744 (+) | Length | 145 a.a. |
| NCBI ID | WP_339169277.1 | Uniprot ID | - |
| Organism | Paenibacillus sp. FSL H8-0282 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Genomic island | 5104402..5142222 | 5136307..5136744 | within | 0 |
Gene organization within MGE regions
Location: 5104402..5142222
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NST94_RS22090 (NST94_22090) | - | 5104402..5105412 (-) | 1011 | WP_036683303.1 | glycoside hydrolase family 130 protein | - |
| NST94_RS22095 (NST94_22095) | - | 5105469..5106305 (-) | 837 | WP_036683306.1 | carbohydrate ABC transporter permease | - |
| NST94_RS22100 (NST94_22100) | - | 5106320..5107222 (-) | 903 | WP_036683309.1 | sugar ABC transporter permease | - |
| NST94_RS22105 (NST94_22105) | - | 5107327..5108709 (-) | 1383 | WP_036683312.1 | extracellular solute-binding protein | - |
| NST94_RS22110 (NST94_22110) | - | 5108882..5110513 (-) | 1632 | WP_339244442.1 | response regulator | - |
| NST94_RS22115 (NST94_22115) | - | 5110517..5112325 (-) | 1809 | WP_036683317.1 | sensor histidine kinase | - |
| NST94_RS22120 (NST94_22120) | - | 5112526..5112813 (+) | 288 | WP_076187406.1 | hypothetical protein | - |
| NST94_RS22125 (NST94_22125) | - | 5112955..5113245 (-) | 291 | WP_076317110.1 | helix-turn-helix transcriptional regulator | - |
| NST94_RS22130 (NST94_22130) | - | 5113450..5113710 (-) | 261 | WP_256717515.1 | hypothetical protein | - |
| NST94_RS22135 (NST94_22135) | - | 5113704..5114126 (-) | 423 | WP_076187410.1 | hypothetical protein | - |
| NST94_RS22140 (NST94_22140) | - | 5114189..5114485 (-) | 297 | WP_339281293.1 | hypothetical protein | - |
| NST94_RS22145 (NST94_22145) | - | 5114545..5114745 (-) | 201 | WP_339281294.1 | hypothetical protein | - |
| NST94_RS22150 (NST94_22150) | - | 5114924..5115421 (-) | 498 | WP_076187414.1 | sigma-70 family RNA polymerase sigma factor | - |
| NST94_RS22155 (NST94_22155) | - | 5115597..5116721 (-) | 1125 | WP_339281295.1 | M56 family metallopeptidase | - |
| NST94_RS22160 (NST94_22160) | - | 5116723..5117091 (-) | 369 | WP_036683325.1 | BlaI/MecI/CopY family transcriptional regulator | - |
| NST94_RS22165 (NST94_22165) | - | 5117395..5118063 (+) | 669 | WP_036683328.1 | hypothetical protein | - |
| NST94_RS22170 (NST94_22170) | - | 5118326..5119918 (-) | 1593 | WP_339281296.1 | sensor domain-containing diguanylate cyclase | - |
| NST94_RS22180 (NST94_22180) | - | 5120594..5121544 (-) | 951 | WP_339281297.1 | DUF4435 domain-containing protein | - |
| NST94_RS22185 (NST94_22185) | - | 5121531..5122880 (-) | 1350 | WP_339281298.1 | AAA family ATPase | - |
| NST94_RS22190 (NST94_22190) | radC | 5123361..5123969 (+) | 609 | WP_339281299.1 | DNA repair protein RadC | - |
| NST94_RS22195 (NST94_22195) | - | 5124159..5124518 (+) | 360 | WP_339281300.1 | helix-turn-helix transcriptional regulator | - |
| NST94_RS22200 (NST94_22200) | - | 5124533..5125477 (+) | 945 | WP_339281301.1 | hypothetical protein | - |
| NST94_RS22205 (NST94_22205) | - | 5125513..5125740 (+) | 228 | WP_339281302.1 | hypothetical protein | - |
| NST94_RS22210 (NST94_22210) | - | 5125815..5125994 (+) | 180 | WP_339281303.1 | hypothetical protein | - |
| NST94_RS22215 (NST94_22215) | - | 5126023..5126151 (+) | 129 | WP_339281304.1 | hypothetical protein | - |
| NST94_RS22220 (NST94_22220) | - | 5126201..5126566 (-) | 366 | WP_339281305.1 | hypothetical protein | - |
| NST94_RS22225 (NST94_22225) | - | 5126563..5127228 (-) | 666 | WP_339281306.1 | hypothetical protein | - |
| NST94_RS22230 (NST94_22230) | - | 5127375..5128439 (-) | 1065 | WP_339281307.1 | hypothetical protein | - |
| NST94_RS22235 (NST94_22235) | - | 5128790..5128990 (+) | 201 | WP_076103801.1 | helix-turn-helix domain-containing protein | - |
| NST94_RS22240 (NST94_22240) | - | 5129071..5130240 (+) | 1170 | WP_339281308.1 | tyrosine-type recombinase/integrase | - |
| NST94_RS22245 (NST94_22245) | - | 5130237..5130431 (+) | 195 | WP_339281309.1 | hypothetical protein | - |
| NST94_RS22255 (NST94_22255) | - | 5130839..5131315 (-) | 477 | WP_248549351.1 | WGxxGxxG-CTERM domain-containing protein | - |
| NST94_RS22260 (NST94_22260) | - | 5131391..5132077 (-) | 687 | WP_339281310.1 | M15 family metallopeptidase | - |
| NST94_RS22265 (NST94_22265) | - | 5132195..5132359 (+) | 165 | WP_169744799.1 | hypothetical protein | - |
| NST94_RS22270 (NST94_22270) | - | 5132356..5132691 (-) | 336 | WP_076142249.1 | YolD-like family protein | - |
| NST94_RS22275 (NST94_22275) | - | 5132906..5133466 (-) | 561 | WP_076128860.1 | hypothetical protein | - |
| NST94_RS22280 (NST94_22280) | - | 5133584..5134072 (+) | 489 | WP_076128858.1 | PH domain-containing protein | - |
| NST94_RS22285 (NST94_22285) | - | 5134145..5135620 (-) | 1476 | WP_339281311.1 | glycosyl hydrolase | - |
| NST94_RS22290 (NST94_22290) | - | 5135790..5136206 (-) | 417 | WP_076103796.1 | cytidine deaminase | - |
| NST94_RS22295 (NST94_22295) | nucA/comI | 5136307..5136744 (+) | 438 | WP_339169277.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| NST94_RS22300 (NST94_22300) | - | 5136826..5136975 (-) | 150 | WP_155288175.1 | hypothetical protein | - |
| NST94_RS22305 (NST94_22305) | - | 5137125..5137301 (+) | 177 | WP_076098563.1 | YjfB family protein | - |
| NST94_RS22310 (NST94_22310) | - | 5137465..5138016 (+) | 552 | WP_036683410.1 | ImmA/IrrE family metallo-endopeptidase | - |
| NST94_RS22315 (NST94_22315) | - | 5138083..5138244 (+) | 162 | WP_155288176.1 | hypothetical protein | - |
| NST94_RS22320 (NST94_22320) | - | 5138610..5138945 (+) | 336 | WP_076192025.1 | DUF3139 domain-containing protein | - |
| NST94_RS22325 (NST94_22325) | - | 5139042..5139179 (+) | 138 | Protein_4400 | phosphomethylpyrimidine synthase ThiC | - |
| NST94_RS22330 (NST94_22330) | - | 5139513..5140007 (+) | 495 | WP_076220021.1 | sigma-70 family RNA polymerase sigma factor | - |
| NST94_RS22335 (NST94_22335) | - | 5140004..5140993 (+) | 990 | WP_339281312.1 | hypothetical protein | - |
| NST94_RS22340 (NST94_22340) | - | 5141719..5142222 (-) | 504 | WP_076192030.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 145 a.a. Molecular weight: 16168.11 Da Isoelectric Point: 4.5847
>NTDB_id=963328 NST94_RS22295 WP_339169277.1 5136307..5136744(+) (nucA/comI) [Paenibacillus sp. FSL H8-0282]
MKVKKWISSLIIVVLLALASYWFEQNGEPTDPSSPSDSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENR
KESLKGIPTKKGYDRDEWPMAMCKEGGIGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK
MKVKKWISSLIIVVLLALASYWFEQNGEPTDPSSPSDSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENR
KESLKGIPTKKGYDRDEWPMAMCKEGGIGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK
Nucleotide
Download Length: 438 bp
>NTDB_id=963328 NST94_RS22295 WP_339169277.1 5136307..5136744(+) (nucA/comI) [Paenibacillus sp. FSL H8-0282]
TTGAAAGTAAAAAAATGGATCTCCAGTCTCATCATTGTTGTATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGG
AGAGCCTACAGACCCCTCGTCTCCATCCGATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCG
CCAAACATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGT
AAAGAGTCCTTAAAAGGGATTCCCACTAAAAAGGGGTATGACCGGGATGAATGGCCAATGGCTATGTGTAAGGAAGGCGG
CATTGGTGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATT
ATGCAGACGGAACGCGGGTAGAATTTATGTTTAAATAA
TTGAAAGTAAAAAAATGGATCTCCAGTCTCATCATTGTTGTATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGG
AGAGCCTACAGACCCCTCGTCTCCATCCGATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCG
CCAAACATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGT
AAAGAGTCCTTAAAAGGGATTCCCACTAAAAAGGGGTATGACCGGGATGAATGGCCAATGGCTATGTGTAAGGAAGGCGG
CATTGGTGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATT
ATGCAGACGGAACGCGGGTAGAATTTATGTTTAAATAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
69.903 |
71.034 |
0.497 |