Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSS59_RS11600 Genome accession   NZ_CP150143
Coordinates   2361463..2362548 (+) Length   361 a.a.
NCBI ID   WP_034771006.1    Uniprot ID   A0A090IWD5
Organism   Caldifermentibacillus hisashii isolate FSL K6-5071     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2356463..2367548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS59_RS11575 (NSS59_11550) - 2356921..2358615 (+) 1695 WP_041846288.1 DEAD/DEAH box helicase -
  NSS59_RS11580 (NSS59_11555) - 2358584..2359387 (+) 804 WP_108898278.1 YqhG family protein -
  NSS59_RS11585 (NSS59_11560) - 2359928..2360098 (-) 171 WP_368993195.1 DUF2759 family protein -
  NSS59_RS11590 (NSS59_11565) - 2360169..2360411 (-) 243 WP_041846286.1 DUF2626 domain-containing protein -
  NSS59_RS11595 (NSS59_11570) - 2360551..2361252 (-) 702 WP_404996049.1 helix-turn-helix transcriptional regulator -
  NSS59_RS11600 (NSS59_11575) comGA 2361463..2362548 (+) 1086 WP_034771006.1 competence type IV pilus ATPase ComGA Machinery gene
  NSS59_RS11605 (NSS59_11580) comGB 2362517..2363554 (+) 1038 WP_404996050.1 competence type IV pilus assembly protein ComGB -
  NSS59_RS11610 (NSS59_11585) comGC 2363581..2363880 (+) 300 WP_034771010.1 competence type IV pilus major pilin ComGC Machinery gene
  NSS59_RS11615 (NSS59_11590) comGD 2363855..2364358 (+) 504 WP_034771013.1 competence type IV pilus minor pilin ComGD -
  NSS59_RS11620 (NSS59_11595) - 2364333..2364689 (+) 357 WP_041846280.1 hypothetical protein -
  NSS59_RS11625 (NSS59_11600) comGF 2364661..2365125 (+) 465 WP_404996051.1 competence type IV pilus minor pilin ComGF -
  NSS59_RS11630 (NSS59_11605) comGG 2365016..2365546 (+) 531 WP_404996052.1 competence type IV pilus minor pilin ComGG -
  NSS59_RS11635 (NSS59_11610) - 2365647..2365832 (+) 186 WP_034771024.1 YqzE family protein -
  NSS59_RS11640 (NSS59_11615) - 2365863..2366987 (-) 1125 WP_404996053.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40615.22 Da        Isoelectric Point: 7.5768

>NTDB_id=963212 NSS59_RS11600 WP_034771006.1 2361463..2362548(+) (comGA) [Caldifermentibacillus hisashii isolate FSL K6-5071]
MPNSIETRAREILEHALSLHATDIHIIPKSKHASVLFRLSHKLIPIMTIELEETERLISYMKFQAAMDIGEKRKPQNGSF
QIDIGGLSVGLRLSTLPSIFAESLVIRILPQESYIPFQQLSLFPKSLRLLLAMLKFSHGLIIFTGPTGSGKTTTLYSLLQ
HSTKSLGRNVITLEDPVEKNSEDLLQVQVNEKAGITYNTGLKAILRHDPDIIMVGEIRDSETAHIAIRAALTGHLVLTTM
HTKDSKGALYRLIEFGVNWHEIEQTLVAVTAQRLVELICPYCLEEECPVYCNQNKNKRTAVYEILYGRALKEALLEMKGE
AFSARYPTLGQLIAKGIALGFIKKSEYERWVHDIETKSLEN

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=963212 NSS59_RS11600 WP_034771006.1 2361463..2362548(+) (comGA) [Caldifermentibacillus hisashii isolate FSL K6-5071]
TTGCCGAACTCGATTGAAACACGGGCAAGGGAAATTTTGGAGCACGCCCTTTCTTTACATGCAACAGATATTCATATCAT
CCCCAAATCAAAACATGCATCCGTTCTATTTCGATTGTCTCATAAACTCATCCCCATAATGACAATCGAACTGGAGGAAA
CGGAAAGATTAATTTCATATATGAAGTTTCAGGCAGCAATGGATATCGGCGAAAAAAGAAAACCCCAGAACGGTTCTTTT
CAAATAGATATCGGGGGGCTATCTGTCGGACTTCGATTATCCACTTTGCCTTCCATATTCGCTGAAAGTTTGGTCATCCG
AATATTACCACAAGAATCATACATCCCATTTCAACAATTATCCTTATTCCCAAAATCACTGAGACTACTGTTAGCCATGT
TAAAGTTTTCCCACGGTTTAATCATATTTACAGGACCAACCGGATCGGGCAAAACAACAACTTTATATTCTCTTCTCCAA
CATTCGACAAAATCTCTTGGCAGGAATGTCATCACCTTGGAAGATCCTGTTGAAAAAAATAGTGAAGATTTACTTCAAGT
TCAAGTAAATGAAAAAGCCGGCATTACGTATAATACCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATTATTATGG
TCGGCGAAATTCGTGATAGTGAGACAGCACATATTGCCATTCGAGCAGCATTAACCGGTCATTTAGTATTGACAACGATG
CATACGAAAGATTCAAAAGGTGCATTGTACCGGTTAATCGAATTTGGTGTTAATTGGCATGAAATTGAACAAACTTTGGT
GGCAGTGACGGCCCAAAGATTAGTCGAATTAATTTGTCCTTACTGTTTAGAAGAAGAATGTCCCGTCTATTGCAACCAGA
ATAAAAATAAGCGTACTGCCGTTTATGAAATTTTGTACGGTCGTGCATTAAAGGAAGCACTGTTGGAAATGAAGGGTGAA
GCATTTTCGGCGCGCTATCCGACTTTGGGCCAATTGATTGCAAAAGGTATTGCTCTCGGATTTATCAAGAAAAGTGAGTA
TGAAAGATGGGTGCATGATATTGAGACAAAGTCGCTGGAAAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A090IWD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.695

97.23

0.551