Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJEHU_RS02375 Genome accession   NZ_CP174221
Coordinates   517201..518595 (-) Length   464 a.a.
NCBI ID   WP_014326638.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF646     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512201..523595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHU_RS02340 (ACJEHU_02340) - 512259..513269 (-) 1011 WP_010946219.1 class I fructose-bisphosphate aldolase -
  ACJEHU_RS02345 (ACJEHU_02345) - 513419..514153 (-) 735 WP_016356729.1 ferredoxin--NADP reductase -
  ACJEHU_RS02350 (ACJEHU_02350) dotV 514237..514779 (+) 543 WP_010946221.1 type IV secretion system protein DotV -
  ACJEHU_RS02355 (ACJEHU_02355) - 514923..515207 (-) 285 WP_011945638.1 DUF3579 domain-containing protein -
  ACJEHU_RS02360 (ACJEHU_02360) pssA 515437..516174 (+) 738 WP_015444818.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACJEHU_RS02365 (ACJEHU_02365) - 516415..516684 (-) 270 WP_010946224.1 HPr family phosphocarrier protein -
  ACJEHU_RS02370 (ACJEHU_02370) hpf 516875..517174 (-) 300 WP_010946225.1 ribosome hibernation promoting factor -
  ACJEHU_RS02375 (ACJEHU_02375) rpoN/rpoN1 517201..518595 (-) 1395 WP_014326638.1 RNA polymerase factor sigma-54 Machinery gene
  ACJEHU_RS02380 (ACJEHU_02380) rpmG 518805..518969 (-) 165 WP_003635328.1 50S ribosomal protein L33 -
  ACJEHU_RS02385 (ACJEHU_02385) rpmB 518984..519220 (-) 237 WP_010946227.1 50S ribosomal protein L28 -
  ACJEHU_RS02390 (ACJEHU_02390) trmB 519489..520166 (+) 678 WP_015444815.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJEHU_RS02395 (ACJEHU_02395) - 520178..521302 (+) 1125 WP_015444814.1 M42 family metallopeptidase -
  ACJEHU_RS02400 (ACJEHU_02400) ankC 521380..522867 (+) 1488 WP_010946231.1 Dot/Icm T4SS effector AnkC/LegA12 -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52953.25 Da        Isoelectric Point: 5.4456

>NTDB_id=962880 ACJEHU_RS02375 WP_014326638.1 517201..518595(-) (rpoN/rpoN1) [Legionella pneumophila strain SPF646]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=962880 ACJEHU_RS02375 WP_014326638.1 517201..518595(-) (rpoN/rpoN1) [Legionella pneumophila strain SPF646]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAACAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACTAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCGCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTCGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCGTTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTAGGATGCGCATCTGTTAATTTAGCAGAAACACTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCCAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTGACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGCCAATATGCCGCTTTAATCC
AACGCGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTGCAAGAAGCACGTTGGTTTTTAAAAAGCATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTCGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAGCGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

39.087

100

0.425