Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WMO33_RS03680 Genome accession   NZ_CP150117
Coordinates   783050..784183 (-) Length   377 a.a.
NCBI ID   WP_024710790.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzicola strain MD-P1-1B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 778050..789183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMO33_RS03660 (WMO33_03660) queD 778491..778844 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  WMO33_RS03665 (WMO33_03665) - 779255..780637 (-) 1383 WP_024710787.1 DEAD/DEAH box helicase -
  WMO33_RS03670 (WMO33_03670) - 780946..782004 (+) 1059 WP_178385910.1 fumarylacetoacetate hydrolase family protein -
  WMO33_RS03675 (WMO33_03675) maiA 782048..782710 (+) 663 WP_024710789.1 maleylacetoacetate isomerase -
  WMO33_RS03680 (WMO33_03680) pilU 783050..784183 (-) 1134 WP_024710790.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WMO33_RS03685 (WMO33_03685) - 784282..784665 (+) 384 WP_033004684.1 DUF4398 domain-containing protein -
  WMO33_RS03690 (WMO33_03690) - 784662..785552 (+) 891 WP_014504659.1 membrane protein -
  WMO33_RS03695 (WMO33_03695) - 785894..786112 (+) 219 WP_003484103.1 YdcH family protein -
  WMO33_RS03700 (WMO33_03700) - 786353..787723 (+) 1371 WP_024710792.1 pyridoxal-phosphate dependent enzyme -
  WMO33_RS03705 (WMO33_03705) - 787814..789010 (+) 1197 WP_024710793.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41867.36 Da        Isoelectric Point: 6.5076

>NTDB_id=962866 WMO33_RS03680 WP_024710790.1 783050..784183(-) (pilU) [Xanthomonas oryzae pv. oryzicola strain MD-P1-1B]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPEAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELHLPQVLKDIIMTPRGLVLVVGSTGSGKSTSLAAMIDHRNGTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGIDGRRRPATEVLLNTPMIRDLLRRGKVHEVKQAMEESLQEGMQTFDQCL
FRMVKSSEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETPAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=962866 WMO33_RS03680 WP_024710790.1 783050..784183(-) (pilU) [Xanthomonas oryzae pv. oryzicola strain MD-P1-1B]
ATGGATATCGGCTACTTCTTGAAGCTGATGACCGAAAAGAATGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGATTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAACTCAACATGGCCATCGCCCTGCCCGAGGCCGGCCGC
TTCCGCGTGAACGTGTTCAAACAGCGCGGCGAGGTAGGCATGGTGATCCGTGCCATTCGCAGCCGGATCCCCAGCATCGA
AGAGCTCCACCTGCCACAGGTGCTCAAGGACATTATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTTGCCGCGATGATCGACCACCGCAACGGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGATACCCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAAGCAG
CGATCGCCTTCGCCGAAACAGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGTATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTCATCTCCCAGCG
TCTGGTCAAGGGCATCGACGGCCGTCGCCGCCCGGCCACCGAAGTGCTGCTCAATACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCAAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGCAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTGAAGTCGAGCGAAATCGAGCAGGAAGAAGCGCTGCGTGCCGCCGACTCGCGTGACGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAAGGCGGCTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGCCGGCTGCGCCGAAGATCAGCC
ACGGGTTTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.718

93.899

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.455

99.204

0.451
  pilT Pseudomonas aeruginosa PAK

43.491

89.655

0.39
  pilT Acinetobacter baumannii D1279779

42.308

89.655

0.379
  pilT Acinetobacter nosocomialis M2

42.308

89.655

0.379
  pilT Acinetobacter baumannii strain A118

42.308

89.655

0.379
  pilT Acinetobacter baylyi ADP1

41.765

90.186

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.216

88.594

0.374
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Neisseria gonorrhoeae MS11

40.058

92.042

0.369
  pilT Neisseria meningitidis 8013

40.058

92.042

0.369
  pilT Vibrio cholerae strain A1552

41.742

88.329

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

88.329

0.369