Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJEIG_RS07995 Genome accession   NZ_CP174209
Coordinates   1782672..1784066 (+) Length   464 a.a.
NCBI ID   WP_014326638.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1777672..1789066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEIG_RS07970 (ACJEIG_07970) ankC 1778400..1779887 (-) 1488 WP_010946231.1 Dot/Icm T4SS effector AnkC/LegA12 -
  ACJEIG_RS07975 (ACJEIG_07975) - 1779965..1781089 (-) 1125 WP_015444814.1 M42 family metallopeptidase -
  ACJEIG_RS07980 (ACJEIG_07980) trmB 1781101..1781778 (-) 678 WP_015444815.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJEIG_RS07985 (ACJEIG_07985) rpmB 1782047..1782283 (+) 237 WP_010946227.1 50S ribosomal protein L28 -
  ACJEIG_RS07990 (ACJEIG_07990) rpmG 1782298..1782462 (+) 165 WP_003635328.1 50S ribosomal protein L33 -
  ACJEIG_RS07995 (ACJEIG_07995) rpoN/rpoN1 1782672..1784066 (+) 1395 WP_014326638.1 RNA polymerase factor sigma-54 Machinery gene
  ACJEIG_RS08000 (ACJEIG_08000) hpf 1784093..1784392 (+) 300 WP_010946225.1 ribosome hibernation promoting factor -
  ACJEIG_RS08005 (ACJEIG_08005) - 1784583..1784852 (+) 270 WP_010946224.1 HPr family phosphocarrier protein -
  ACJEIG_RS08010 (ACJEIG_08010) pssA 1785093..1785830 (-) 738 WP_015444818.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACJEIG_RS08015 (ACJEIG_08015) - 1786060..1786344 (+) 285 WP_011945638.1 DUF3579 domain-containing protein -
  ACJEIG_RS08020 (ACJEIG_08020) dotV 1786488..1787030 (-) 543 WP_010946221.1 type IV secretion system protein DotV -
  ACJEIG_RS08025 (ACJEIG_08025) - 1787114..1787848 (+) 735 WP_016356729.1 ferredoxin--NADP reductase -
  ACJEIG_RS08030 (ACJEIG_08030) - 1787998..1789008 (+) 1011 WP_010946219.1 class I fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52953.25 Da        Isoelectric Point: 5.4456

>NTDB_id=962091 ACJEIG_RS07995 WP_014326638.1 1782672..1784066(+) (rpoN/rpoN1) [Legionella pneumophila strain SPF666]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=962091 ACJEIG_RS07995 WP_014326638.1 1782672..1784066(+) (rpoN/rpoN1) [Legionella pneumophila strain SPF666]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAACAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACTAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCGCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTCGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCGTTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTAGGATGCGCATCTGTTAATTTAGCAGAAACACTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCCAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTGACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGCCAATATGCCGCTTTAATCC
AACGCGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTGCAAGAAGCACGTTGGTTTTTAAAAAGCATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGAAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTCGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAGCGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

39.087

100

0.425