Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WKU38_RS20330 Genome accession   NZ_CP149853
Coordinates   4322655..4324088 (+) Length   477 a.a.
NCBI ID   WP_339275470.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 2024CK-00035     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4317655..4329088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU38_RS20300 (WKU38_20300) - 4318222..4319199 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  WKU38_RS20305 (WKU38_20305) kdsD 4319213..4320199 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  WKU38_RS20310 (WKU38_20310) kdsC 4320214..4320780 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WKU38_RS20315 (WKU38_20315) lptC 4320777..4321352 (+) 576 WP_121424777.1 LPS export ABC transporter periplasmic protein LptC -
  WKU38_RS20320 (WKU38_20320) lptA 4321321..4321875 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WKU38_RS20325 (WKU38_20325) lptB 4321882..4322607 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  WKU38_RS20330 (WKU38_20330) rpoN/rpoN1 4322655..4324088 (+) 1434 WP_339275470.1 RNA polymerase factor sigma-54 Machinery gene
  WKU38_RS20335 (WKU38_20335) hpf 4324111..4324398 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  WKU38_RS20340 (WKU38_20340) ptsN 4324481..4324972 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WKU38_RS20345 (WKU38_20345) rapZ 4325018..4325872 (+) 855 WP_014885397.1 RNase adapter RapZ -
  WKU38_RS20350 (WKU38_20350) npr 4325869..4326141 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  WKU38_RS20355 (WKU38_20355) mtgA 4326179..4326904 (-) 726 WP_121424779.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WKU38_RS20360 (WKU38_20360) elbB 4326901..4327554 (-) 654 WP_121424780.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54211.98 Da        Isoelectric Point: 4.3198

>NTDB_id=961978 WKU38_RS20330 WP_339275470.1 4322655..4324088(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2024CK-00035]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNERWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=961978 WKU38_RS20330 WP_339275470.1 4322655..4324088(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2024CK-00035]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCTAATCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGACGTGCTGGTCAGAAAACACAATGAGCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482