Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   WKU69_RS17840 Genome accession   NZ_CP149838
Coordinates   3670960..3671700 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain 2024CK-00130     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3665960..3676700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU69_RS17820 (WKU69_17820) aroB 3666502..3667584 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  WKU69_RS17825 (WKU69_17825) aroK 3667613..3668182 (-) 570 WP_000643712.1 shikimate kinase AroK -
  WKU69_RS17830 (WKU69_17830) pilQ 3668194..3670359 (-) 2166 WP_001017040.1 type IV pilus secretin PilQ family protein Machinery gene
  WKU69_RS17835 (WKU69_17835) pilP 3670422..3670949 (-) 528 WP_000695059.1 pilus assembly protein PilP Machinery gene
  WKU69_RS17840 (WKU69_17840) pilO 3670960..3671700 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  WKU69_RS17845 (WKU69_17845) pilN 3671697..3672338 (-) 642 WP_032017526.1 PilN domain-containing protein Machinery gene
  WKU69_RS17850 (WKU69_17850) pilM 3672338..3673396 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  WKU69_RS17855 (WKU69_17855) ponA 3673558..3676113 (+) 2556 WP_000736679.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=961892 WKU69_RS17840 WP_000076101.1 3670960..3671700(-) (pilO) [Acinetobacter baumannii strain 2024CK-00130]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=961892 WKU69_RS17840 WP_000076101.1 3670960..3671700(-) (pilO) [Acinetobacter baumannii strain 2024CK-00130]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683