Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   WKU69_RS02445 Genome accession   NZ_CP149838
Coordinates   524720..525520 (+) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 2024CK-00130     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 519720..530520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU69_RS02420 (WKU69_02420) - 520630..521928 (+) 1299 WP_001991191.1 glycosyltransferase family 1 protein -
  WKU69_RS02425 (WKU69_02425) - 521953..522504 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  WKU69_RS02430 (WKU69_02430) iscX 522604..522804 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  WKU69_RS02435 (WKU69_02435) ndk 522918..523349 (+) 432 WP_339242930.1 nucleoside-diphosphate kinase -
  WKU69_RS02440 (WKU69_02440) rlmN 523472..524704 (+) 1233 WP_339242933.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  WKU69_RS02445 (WKU69_02445) pilF 524720..525520 (+) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  WKU69_RS02450 (WKU69_02450) - 525511..526302 (+) 792 WP_000405163.1 RodZ domain-containing protein -
  WKU69_RS02455 (WKU69_02455) ispG 526324..527439 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  WKU69_RS02460 (WKU69_02460) hisS 527436..528728 (+) 1293 WP_000095263.1 histidine--tRNA ligase -
  WKU69_RS02465 (WKU69_02465) - 528757..529455 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=961865 WKU69_RS02445 WP_001984465.1 524720..525520(+) (pilF) [Acinetobacter baumannii strain 2024CK-00130]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=961865 WKU69_RS02445 WP_001984465.1 524720..525520(+) (pilF) [Acinetobacter baumannii strain 2024CK-00130]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722