Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WKV34_RS20265 Genome accession   NZ_CP149825
Coordinates   4196728..4198161 (+) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain 2024CK-00243     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4191728..4203161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKV34_RS20235 (WKV34_20235) - 4192295..4193272 (+) 978 WP_045630730.1 calcium/sodium antiporter -
  WKV34_RS20240 (WKV34_20240) kdsD 4193286..4194272 (+) 987 WP_028014567.1 arabinose-5-phosphate isomerase KdsD -
  WKV34_RS20245 (WKV34_20245) kdsC 4194287..4194853 (+) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WKV34_RS20250 (WKV34_20250) lptC 4194850..4195425 (+) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  WKV34_RS20255 (WKV34_20255) lptA 4195394..4195948 (+) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WKV34_RS20260 (WKV34_20260) lptB 4195955..4196680 (+) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  WKV34_RS20265 (WKV34_20265) rpoN/rpoN1 4196728..4198161 (+) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  WKV34_RS20270 (WKV34_20270) hpf 4198184..4198471 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  WKV34_RS20275 (WKV34_20275) ptsN 4198554..4199045 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WKV34_RS20280 (WKV34_20280) rapZ 4199091..4199945 (+) 855 WP_014885397.1 RNase adapter RapZ -
  WKV34_RS20285 (WKV34_20285) npr 4199942..4200214 (+) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  WKV34_RS20290 (WKV34_20290) mtgA 4200253..4200978 (-) 726 WP_028014572.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WKV34_RS20295 (WKV34_20295) elbB 4200975..4201628 (-) 654 WP_047364720.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=961749 WKV34_RS20265 WP_028014571.1 4196728..4198161(+) (rpoN/rpoN1) [Enterobacter bugandensis strain 2024CK-00243]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=961749 WKV34_RS20265 WP_028014571.1 4196728..4198161(+) (rpoN/rpoN1) [Enterobacter bugandensis strain 2024CK-00243]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACTGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCCATTGCCACCTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCTAAAGACCTGCGCGACTGTTTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48