Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACIX56_RS05640 Genome accession   NZ_CP173676
Coordinates   1235035..1237251 (-) Length   738 a.a.
NCBI ID   WP_012478474.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APL2303082E     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230035..1242251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIX56_RS05620 (ACIX56_05630) - 1231122..1231892 (-) 771 WP_005601479.1 glycosyltransferase family 25 protein -
  ACIX56_RS05625 (ACIX56_05635) - 1231889..1233097 (-) 1209 WP_005597816.1 lipopolysaccharide biosynthesis protein -
  ACIX56_RS05630 (ACIX56_05640) - 1233075..1234040 (-) 966 WP_005597818.1 polysaccharide pyruvyl transferase family protein -
  ACIX56_RS05635 (ACIX56_05645) - 1234196..1234936 (+) 741 WP_005610311.1 YdcF family protein -
  ACIX56_RS05640 (ACIX56_05650) priA 1235035..1237251 (-) 2217 WP_012478474.1 primosomal protein N' Machinery gene
  ACIX56_RS05645 (ACIX56_05655) - 1237335..1238492 (-) 1158 WP_005617512.1 chorismate mutase -
  ACIX56_RS05650 (ACIX56_05660) prlC 1238615..1240648 (-) 2034 WP_005610315.1 oligopeptidase A -
  ACIX56_RS05655 (ACIX56_05665) pflA 1240791..1241528 (-) 738 WP_405200726.1 pyruvate formate lyase 1-activating protein -

Sequence

Protein


Download         Length: 738 a.a.        Molecular weight: 83304.02 Da        Isoelectric Point: 8.7569

>NTDB_id=961683 ACIX56_RS05640 WP_012478474.1 1235035..1237251(-) (priA) [Actinobacillus pleuropneumoniae strain APL2303082E]
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVAVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTVTEAGRAALLSGENKRAKKQQDLLAKLAEIANFFEKPTACS
QSAWKGLLEKNYIEPVNVPFELQHWQKKLQNRPLVNLHNRLVLNKQQTLVVSRLAVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWWRAKNGESAIVIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKQHLEQGNQVMLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
GLQNNIRLTLDIDPQEIG

Nucleotide


Download         Length: 2217 bp        

>NTDB_id=961683 ACIX56_RS05640 WP_012478474.1 1235035..1237251(-) (priA) [Actinobacillus pleuropneumoniae strain APL2303082E]
ATGTCGAAGCTAGTTAGAGTGGCATTACCCGTGCCGTTACATCGTTATTTTGATTATTTACTGCCGCCTTTTTTGTCGGC
GGTCAAAGGCGTGCGTGTTGAAGTGCCTTTCGGTAATCAAACGAAAATCGGCGTGGCGGTGGATTTCCCTGAAACAAGTG
ATGTGCCTGAAGATAAGCTCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGACGAAGAAATTTGGACACTG
CTTCGTTGGGCGGCTCGTTATTATCATGCGCCTATCGGCGAAGTGGCGATCAATGCTTTGCCGGTTAAATTACGTAACGG
TGATAGTACCGAAAGATCGCAACCGGATTACTTTACTGTTACTGAGGCAGGGCGGGCGGCATTGCTTTCGGGTGAGAACA
AGCGGGCCAAAAAACAGCAAGATTTACTGGCGAAACTGGCAGAAATTGCAAATTTTTTTGAAAAACCGACCGCTTGTAGT
CAATCGGCTTGGAAAGGGCTATTGGAAAAAAACTATATCGAACCGGTTAATGTGCCGTTTGAGCTGCAACATTGGCAAAA
AAAATTGCAAAATCGACCGCTTGTAAACTTGCATAATCGACTGGTATTAAATAAACAGCAAACCCTAGTGGTGAGCCGCT
TAGCAGTACAAAACGGTTTCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCAGGCAAAACCGAAGTGTATTTGCAAGTG
ATCGAAGAGGTGTTAAAGCGAGGCGAGCAAGTATTGGTGTTAGTGCCGGAAATCGGGCTGACTCCGCAAACGGTGCAACG
TTTTAAAGCCCGTTTTAATGTTGAAATTGATGTTCTTCATTCGAATATGAATGAAACAGAGCGATTAAATGCTTGGTGGC
GAGCAAAAAACGGCGAAAGTGCGATTGTGATCGGCACGCGTTCGGCATTGTTCAGCCAGTTTGAGAAATTGGGCTTAATC
ATTATTGATGAAGAACACGATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCGATTTTACG
GGCAAAAAATTTACATATCCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTA
AGTTTATTGAGCTATCTTTAACCGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAG
CGTATTACCGCCGGACTTTCCGACCGCTTATTAGCGATGATGAAGCAGCATTTAGAGCAAGGCAATCAGGTAATGTTGTT
TTTAAATCGCCGCGGCTTTGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGC
CTTACACTTATCATCAGAAACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGG
CATTGCGGCTCAACCAATCTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAAC
TTACCAAATTAGCCGTATCGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAACCACTTAAATGATATCCGAGAAG
GTAAAAGCCAAATCCTTATCGGCACGCAAATGTTAGCGAAAGGGCATCATTTTCCGAATGTAACGTTAGTGGCGATTGTG
AATGTAGATTCGGCACTGTTTTCGACCGACTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACG
TGCCGGACGAGCGGAAAAGCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAG
AACAAGGTTATTTGGCGTTTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCTGCGCAAGTG
TTGTTACGGGCAACCGGCAAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATG
TACGGAACTCGGTTTGCAGGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCT
GGCTTTTATTAATTCAACATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTTGGATAAAGCCAATTTA
GGCTTACAAAATAATATTCGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(221-369)

Primosomal protein N C-terminal domain (PriA_C)

(635-734)

Helicase conserved C-terminal domain (Helicase_C)

(497-591)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(448-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.28

100

0.436