Detailed information NTG0961627

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACI5E1_RS05475
• Genome accession: NZ_CP173662
• Coordinates: 1222065..1224281 (-)
• Length: 738 a.a.
• NCBI ID: WP_237593236.1
• Uniprot ID: -
• Organism: Actinobacillus pleuropneumoniae strain APL2305174E
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1217065..1229281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI5E1_RS05455 (ACI5E1_05450)	-	1218171..1218941 (-)	771	WP_005612388.1	glycosyltransferase family 25 protein	-
ACI5E1_RS05460 (ACI5E1_05455)	-	1218938..1220146 (-)	1209	WP_005604749.1	lipopolysaccharide biosynthesis protein	-
ACI5E1_RS05465 (ACI5E1_05460)	-	1220124..1221089 (-)	966	WP_005604751.1	polysaccharide pyruvyl transferase family protein	-
ACI5E1_RS05470 (ACI5E1_05465)	-	1221245..1221985 (+)	741	WP_215578004.1	YdcF family protein	-
ACI5E1_RS05475 (ACI5E1_05470)	priA	1222065..1224281 (-)	2217	WP_237593236.1	primosomal protein N'	Machinery gene
ACI5E1_RS05480 (ACI5E1_05475)	-	1224366..1225523 (-)	1158	WP_012263072.1	chorismate mutase	-
ACI5E1_RS05485 (ACI5E1_05480)	prlC	1225646..1227679 (-)	2034	WP_237593237.1	oligopeptidase A	-
ACI5E1_RS05490 (ACI5E1_05485)	pflA	1227820..1228557 (-)	738	WP_012263074.1	pyruvate formate lyase 1-activating protein	-

Sequence

Protein

• Length: 738 a.a.
• Molecular weight: 83196.95 Da
• Isoelectric Point: 8.9843

>NTDB_id=961627 ACI5E1_RS05475 WP_237593236.1 1222065..1224281(-) (priA) [Actinobacillus pleuropneumoniae strain APL2305174E]
MSKLVRVALPVPLHRYFDYLLPSFLSAVKGVRVEVPFGNQTKIGIVVDFPETSDVPEDKLKPIKAVLDLEPIFDKEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFAVTEAGRAALLSGENKRAKKQQDLLAKLAEIANFFEKPAACS
QSAWKGLLEKNYIELVNVPFEPQHWQKKLQNRPLVNLQNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIIIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKQHLEQGNQVMLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDSDKANL
GLQNNIRLTLDIDPQEIG

Nucleotide

• Length: 2217 bp

>NTDB_id=961627 ACI5E1_RS05475 WP_237593236.1 1222065..1224281(-) (priA) [Actinobacillus pleuropneumoniae strain APL2305174E]
ATGTCGAAGCTAGTTAGAGTGGCATTGCCCGTGCCGTTACATCGTTATTTTGATTATTTACTGCCGTCGTTTTTGTCGGC
AGTCAAAGGTGTGCGTGTTGAAGTGCCTTTCGGTAATCAAACGAAAATCGGGATTGTAGTGGATTTTCCCGAAACGAGCG
ATGTGCCTGAAGATAAGCTCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGACAAGGAAATTTGGACACTG
CTTCGTTGGGCGGCTCGTTATTATCATGCGCCTATCGGCGAAGTGGCGATCAATGCCTTGCCGGTGAAATTGCGTAACGG
CGATAGTACCGAAAGATCGCAACCGGATTACTTTGCGGTTACTGAGGCAGGGCGAGCAGCATTGCTCTCGGGTGAGAACA
AGCGGGCCAAAAAACAGCAAGATTTACTGGCGAAACTGGCAGAAATTGCAAATTTTTTTGAAAAACCGGCCGCTTGTAGC
CAATCGGCTTGGAAAGGGCTATTGGAAAAAAACTATATCGAACTGGTTAATGTGCCGTTTGAACCGCAACATTGGCAAAA
AAAATTGCAAAATCGACCGCTTGTAAATTTGCAGAATCGACTGGTATTAAATAAACAGCAAAGCTTAGTGGTGAGCCGCT
TAACAGTACAGAACGGTTTCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCGGGCAAAACCGAAGTGTATCTGCAAGTG
ATTGAAGAGGTGTTAAAGCGAGGCGAGCAAGTGTTGGTGTTAGTGCCGGAAATCGGACTGACCCCGCAAACGGTACAACG
ATTTAAAGCCCGTTTTAATGTTGAAATTGACGTGCTGCATTCGAATATGAATGAAACCGAACGCTTGAATGCTTGGTTGC
GAGCAAAAAACGGTGAAAGTGCGATTATCATCGGTACTCGTTCGGCATTGTTTAGCCAGTTTGAGAAATTGGGCTTAATC
ATTATTGATGAAGAACACGATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCAATTTTACG
GGCAAAAAATTTACATATCCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTA
AGTTTATTGAGCTATCTTTAACCGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAG
CGTATTACCGCCGGACTTTCCGACCGCTTATTAGCGATGATGAAGCAGCATTTAGAGCAAGGCAATCAGGTAATGTTGTT
TTTAAATCGCCGCGGCTTTGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGC
CTTACACTTATCATCAGAAACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGG
CATTGCGGCTCAACCAATCTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAAC
TTACCAAATTAGCCGTATCGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAATCACTTAAACGATATCCGAGAAG
GCAAAAGCCAAATTTTGATTGGCACGCAAATGCTAGCGAAAGGACACCATTTTCCGAATGTAACTTTAGTGGCGATTGTG
AATGTAGATTCGGCACTGTTTTCGACCGACTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACG
TGCCGGACGGGCGGAAAAGCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAG
AACAAGGTTATTTGGCGTTTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCCGCACAAGTG
TTGTTACGGGCAACCGGCAAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATG
TACGGAACTCGGTTTGCAGGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCT
GGCTTTTATTAATTCAACATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTCGGATAAAGCCAATTTA
GGCTTACAAAATAATATTCGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (221-369)

• Helicase conserved C-terminal domain (Helicase_C) (497-591)

• Primosomal protein N C-terminal domain (PriA_C) (635-734)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (448-473)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.548	100	0.439