Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCZ21_RS12935 Genome accession   NZ_AP026375
Coordinates   2823773..2824909 (+) Length   378 a.a.
NCBI ID   WP_244073245.1    Uniprot ID   -
Organism   Aeromonas caviae strain KAM497     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2818773..2829909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCZ21_RS12905 (KAM497c_24910) - 2818879..2819259 (-) 381 WP_052816484.1 diacylglycerol kinase -
  OCZ21_RS12910 (KAM497c_24920) - 2819401..2820465 (+) 1065 WP_113977416.1 3-deoxy-7-phosphoheptulonate synthase -
  OCZ21_RS12915 (KAM497c_24930) - 2820673..2821446 (-) 774 WP_202016592.1 ATP-binding cassette domain-containing protein -
  OCZ21_RS12920 (KAM497c_24940) btuC 2821433..2822440 (-) 1008 WP_104453101.1 vitamin B12 ABC transporter permease BtuC -
  OCZ21_RS12925 (KAM497c_24950) - 2822516..2823247 (-) 732 WP_104453102.1 ABC transporter substrate-binding protein -
  OCZ21_RS12930 (KAM497c_24960) - 2823360..2823716 (+) 357 WP_010675200.1 DMT family protein -
  OCZ21_RS12935 (KAM497c_24970) pilU 2823773..2824909 (+) 1137 WP_244073245.1 type IVa pilus ATPase TapW Machinery gene
  OCZ21_RS12940 (KAM497c_24980) - 2824971..2825213 (-) 243 WP_010675202.1 FeoC-like transcriptional regulator -
  OCZ21_RS12945 (KAM497c_24990) feoB 2825260..2827530 (-) 2271 WP_244073243.1 Fe(2+) transporter permease subunit FeoB -
  OCZ21_RS12950 (KAM497c_25000) - 2827527..2827754 (-) 228 WP_010675204.1 FeoA family protein -
  OCZ21_RS12955 (KAM497c_25010) - 2827874..2828203 (+) 330 WP_039041208.1 translation initiation factor -
  OCZ21_RS12960 (KAM497c_25020) - 2828457..2829014 (+) 558 WP_010675206.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42217.29 Da        Isoelectric Point: 6.6000

>NTDB_id=96151 OCZ21_RS12935 WP_244073245.1 2823773..2824909(+) (pilU) [Aeromonas caviae strain KAM497]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAALEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=96151 OCZ21_RS12935 WP_244073245.1 2823773..2824909(+) (pilU) [Aeromonas caviae strain KAM497]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
TGCCAAGTTCAACGGCGCCCTGCGCCCCCTTACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAGTCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGCTGGAGGTGATGCTCGGAACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGTACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.235

94.709

0.495
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.515

88.36

0.376
  pilT Legionella pneumophila strain Lp02

42.515

88.36

0.376
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment