Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   WKW51_RS15645 Genome accession   NZ_CP149800
Coordinates   3261179..3261979 (-) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AB7276     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3256179..3266979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKW51_RS15625 (WKW51_15625) - 3257244..3257942 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  WKW51_RS15630 (WKW51_15630) hisS 3257971..3259263 (-) 1293 WP_000095263.1 histidine--tRNA ligase -
  WKW51_RS15635 (WKW51_15635) ispG 3259260..3260375 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  WKW51_RS15640 (WKW51_15640) - 3260397..3261188 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  WKW51_RS15645 (WKW51_15645) pilF 3261179..3261979 (-) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  WKW51_RS15650 (WKW51_15650) rlmN 3261995..3263227 (-) 1233 WP_031959661.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  WKW51_RS15655 (WKW51_15655) ndk 3263350..3263781 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  WKW51_RS15660 (WKW51_15660) iscX 3263895..3264095 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  WKW51_RS15665 (WKW51_15665) - 3264195..3264746 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  WKW51_RS15670 (WKW51_15670) - 3264771..3266069 (-) 1299 WP_001991191.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=961430 WKW51_RS15645 WP_002027454.1 3261179..3261979(-) (pilF) [Acinetobacter baumannii strain AB7276]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=961430 WKW51_RS15645 WP_002027454.1 3261179..3261979(-) (pilF) [Acinetobacter baumannii strain AB7276]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726