Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   ACI3ML_RS04815 Genome accession   NZ_CP173596
Coordinates   652470..653462 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain 98192     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 647470..658462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3ML_RS04790 (ACI3ML_04790) ygbN 648125..649489 (+) 1365 WP_000104455.1 GntP family transporter -
  ACI3ML_RS04795 (ACI3ML_04795) - 649528..649764 (-) 237 WP_000562982.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  ACI3ML_RS04800 (ACI3ML_04800) - 649775..651202 (-) 1428 WP_000863205.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  ACI3ML_RS04805 (ACI3ML_04805) - 651202..651795 (-) 594 WP_001521150.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  ACI3ML_RS04810 (ACI3ML_04810) - 651942..652349 (+) 408 WP_001521151.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACI3ML_RS04815 (ACI3ML_04815) rpoS 652470..653462 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  ACI3ML_RS04820 (ACI3ML_04820) nlpD 653525..654619 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  ACI3ML_RS04825 (ACI3ML_04825) pcm 654804..655430 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  ACI3ML_RS04830 (ACI3ML_04830) surE 655424..656185 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  ACI3ML_RS04835 (ACI3ML_04835) truD 656166..657215 (-) 1050 WP_000568924.1 tRNA pseudouridine(13) synthase TruD -
  ACI3ML_RS04840 (ACI3ML_04840) ispF 657212..657691 (-) 480 WP_001521152.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACI3ML_RS04845 (ACI3ML_04845) ispD 657691..658401 (-) 711 WP_000246138.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=961358 ACI3ML_RS04815 WP_000081550.1 652470..653462(-) (rpoS) [Escherichia coli strain 98192]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=961358 ACI3ML_RS04815 WP_000081550.1 652470..653462(-) (rpoS) [Escherichia coli strain 98192]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTTGCCTCCCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGCGAGTTGTCCCA
TAAACTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715