Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACI3MS_RS25130 Genome accession   NZ_CP173588
Coordinates   4748321..4749703 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain 98194     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4743321..4754703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3MS_RS25110 (ACI3MS_25095) ettA 4744635..4746302 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  ACI3MS_RS25115 (ACI3MS_25100) nadS 4746358..4746642 (-) 285 WP_000007436.1 NadS family protein -
  ACI3MS_RS25120 (ACI3MS_25105) - 4746644..4746976 (-) 333 WP_001545808.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACI3MS_RS25125 (ACI3MS_25110) nadR 4747068..4748300 (-) 1233 WP_000093833.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACI3MS_RS25130 (ACI3MS_25115) radA/sms 4748321..4749703 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  ACI3MS_RS25135 (ACI3MS_25120) serB 4749752..4750720 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  ACI3MS_RS25140 (ACI3MS_25125) ytjB 4750826..4751470 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  ACI3MS_RS25145 (ACI3MS_25130) lplA 4751498..4752514 (+) 1017 WP_000105840.1 lipoate--protein ligase LplA -
  ACI3MS_RS25150 (ACI3MS_25135) - 4752546..4752809 (+) 264 WP_000566247.1 helix-turn-helix domain-containing protein -
  ACI3MS_RS25155 (ACI3MS_25140) deoD 4752970..4753689 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=961260 ACI3MS_RS25130 WP_001029698.1 4748321..4749703(-) (radA/sms) [Escherichia coli strain 98194]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=961260 ACI3MS_RS25130 WP_001029698.1 4748321..4749703(-) (radA/sms) [Escherichia coli strain 98194]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTTTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTCAAAGAGTTCGACCGCGTATTAGGTGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGAGCCGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTCGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTCATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCACCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCAGGCCCTAAAGTGCTGGAACACTGTATCGACTGCTCG
GTGCTTCTGGACGGCGATGCCGACTCCCGTTTCCGCACCTTACGCAGCCATAAAAACCGTTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACTGAACAGGGACTGCGTGAAGTCAGTAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGCAGCTCAGTGATGGTGGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAGCAAAACCGTTTGGCGATCCTGCTGGCGGTGTTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGTGCCG
ATTTAGCGTTACTACTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAGCGGATTTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCAGCCAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43