Detailed information NTG0960456

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WKI55_RS19280
• Genome accession: NZ_CP149651
• Coordinates: 3742203..3743348 (+)
• Length: 381 a.a.
• NCBI ID: WP_252052939.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain MJ01
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3737203..3748348

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI55_RS19260 (WKI55_19260)	fnr	3737680..3738396 (-)	717	WP_252052937.1	fumarate/nitrate reduction transcriptional regulator Fnr	-
WKI55_RS19265 (WKI55_19265)	narK	3738492..3739682 (-)	1191	WP_017418712.1	nitrate transporter NarK	-
WKI55_RS19270 (WKI55_19270)	argS	3739816..3741486 (-)	1671	WP_017418711.1	arginine--tRNA ligase	-
WKI55_RS19275 (WKI55_19275)	-	3741483..3741911 (-)	429	WP_003151069.1	DUF1934 family protein	-
WKI55_RS19280 (WKI55_19280)	rapF	3742203..3743348 (+)	1146	WP_252052939.1	aspartate phosphatase	Regulator
WKI55_RS19285 (WKI55_19285)	-	3743332..3743451 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
WKI55_RS19290 (WKI55_19290)	speB	3743574..3744446 (-)	873	WP_003151054.1	agmatinase	-
WKI55_RS19295 (WKI55_19295)	speE	3744506..3745336 (-)	831	WP_017418706.1	spermidine synthase	-
WKI55_RS19300 (WKI55_19300)	-	3745536..3747608 (+)	2073	WP_017418705.1	transglycosylase domain-containing protein	-
WKI55_RS19305 (WKI55_19305)	-	3747633..3748064 (-)	432	WP_017418704.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45796.32 Da
• Isoelectric Point: 5.0749

>NTDB_id=960456 WKI55_RS19280 WP_252052939.1 3742203..3743348(+) (rapF) [Bacillus velezensis strain MJ01]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDEKKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLENIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLESKLMYADIEELALDVAKYYYTKEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=960456 WKI55_RS19280 WP_252052939.1 3742203..3743348(+) (rapF) [Bacillus velezensis strain MJ01]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGAAAAAAAATACAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGAACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGCCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAGAAAATATAGATAATGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTGGAATATTTATATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAGTAAACTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACAAAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAAGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	53.684	99.738	0.535