Detailed information NTG0960386

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WKI56_RS18345
• Genome accession: NZ_CP149650
• Coordinates: 3614862..3616007 (+)
• Length: 381 a.a.
• NCBI ID: WP_017418708.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain MJ02
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3609862..3621007

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI56_RS18325 (WKI56_18325)	fnr	3610337..3611053 (-)	717	WP_017418713.1	fumarate/nitrate reduction transcriptional regulator Fnr	-
WKI56_RS18330 (WKI56_18330)	narK	3611151..3612341 (-)	1191	WP_017418712.1	nitrate transporter NarK	-
WKI56_RS18335 (WKI56_18335)	argS	3612475..3614145 (-)	1671	WP_017418711.1	arginine--tRNA ligase	-
WKI56_RS18340 (WKI56_18340)	-	3614142..3614570 (-)	429	WP_003151069.1	DUF1934 family protein	-
WKI56_RS18345 (WKI56_18345)	rapF	3614862..3616007 (+)	1146	WP_017418708.1	tetratricopeptide repeat protein	Regulator
WKI56_RS18350 (WKI56_18350)	-	3615991..3616110 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
WKI56_RS18355 (WKI56_18355)	speB	3616233..3617105 (-)	873	WP_003151054.1	agmatinase	-
WKI56_RS18360 (WKI56_18360)	speE	3617165..3617995 (-)	831	WP_276787310.1	spermidine synthase	-
WKI56_RS18365 (WKI56_18365)	-	3618195..3620267 (+)	2073	WP_167860509.1	transglycosylase domain-containing protein	-
WKI56_RS18370 (WKI56_18370)	-	3620292..3620723 (-)	432	WP_110085370.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45782.29 Da
• Isoelectric Point: 5.0722

>NTDB_id=960386 WKI56_RS18345 WP_017418708.1 3614862..3616007(+) (rapF) [Bacillus velezensis strain MJ02]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDEKKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLENIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLESKLMYADIEELALDVAKYYYTKEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=960386 WKI56_RS18345 WP_017418708.1 3614862..3616007(+) (rapF) [Bacillus velezensis strain MJ02]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGAAAAAAAATACAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGAACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGCCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAGAAAATATAGATAATGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTGGAATATTTATATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAGTAAACTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACAAAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAAGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.341	100	0.703
rapC	Bacillus subtilis subsp. subtilis str. 168	53.421	99.738	0.533