Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   WKI54_RS20215 Genome accession   NZ_CP149648
Coordinates   3931239..3932039 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain MJ04     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 3926239..3937039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS20185 (WKI54_20185) - 3926781..3927422 (+) 642 WP_339075769.1 helix-turn-helix domain-containing protein -
  WKI54_RS20190 (WKI54_20190) - 3927664..3927846 (+) 183 WP_170616540.1 hypothetical protein -
  WKI54_RS20195 (WKI54_20195) - 3927940..3929058 (+) 1119 WP_025649857.1 NADH-dependent flavin oxidoreductase -
  WKI54_RS20200 (WKI54_20200) - 3929185..3929397 (+) 213 WP_029973891.1 spore germination protein -
  WKI54_RS20205 (WKI54_20205) - 3929523..3929735 (+) 213 WP_014418351.1 spore germination protein -
  WKI54_RS20210 (WKI54_20210) alr 3929910..3931091 (+) 1182 WP_017418123.1 alanine racemase -
  WKI54_RS20215 (WKI54_20215) spo0A 3931239..3932039 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  WKI54_RS20220 (WKI54_20220) spoIVB 3932320..3933600 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  WKI54_RS20225 (WKI54_20225) recN 3933764..3935493 (-) 1730 Protein_3928 DNA repair protein RecN -
  WKI54_RS20230 (WKI54_20230) argR 3935525..3935974 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  WKI54_RS20235 (WKI54_20235) - 3936071..3936916 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=960302 WKI54_RS20215 WP_003153177.1 3931239..3932039(-) (spo0A) [Bacillus velezensis strain MJ04]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=960302 WKI54_RS20215 WP_003153177.1 3931239..3932039(-) (spo0A) [Bacillus velezensis strain MJ04]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGTGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGGGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97